Model lipid vesicles self-reproduce to generate unilamellar daughter vesicles in the presence of a cell-penetrating peptide. Fluorescence lifetime and anisotropy image analysis exquisitely capture the change in the lipid bilayer rigidity throughout the process, whereas a solvation dynamic study delineates the change in the dynamics of the encapsulated water inside the vesicles. Overall, our results provide an experimental underpinning of the role of the lipid bilayer structural changes in self-reproduction, which can exhibit great potential for interpreting the protein–membrane interaction in the emergence of life and for developing new therapeutic strategies.

中文翻译：

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穿透细胞的肽诱导磷脂囊泡的自我繁殖：了解双层刚性的作用†

在细胞穿透肽存在的情况下，模型脂质囊泡会自我繁殖以生成单层子囊泡。荧光寿命和各向异性图像分析可以很好地捕获整个过程中脂质双层刚性的变化，而溶剂化动力学研究则描述了囊泡内部包囊水动力学的变化。总的来说，我们的结果为脂质双层结构变化在自我复制中的作用提供了实验基础，这可能在解释生命出现中的蛋白质-膜相互作用以及开发新的治疗策略方面显示出巨大的潜力。