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How Entanglement of Different Physicochemical Properties Complicates the Prediction of in Vitro and in Vivo Interactions of Gold Nanoparticles
ACS Nano ( IF 17.1 ) Pub Date : 2018-09-13 00:00:00 , DOI: 10.1021/acsnano.8b04906
Ming Xu 1, 2 , Mahmoud G. Soliman 3, 4, 5 , Xing Sun 3, 4 , Beatriz Pelaz 3 , Neus Feliu 4, 6 , Wolfgang J. Parak 3, 4 , Sijin Liu 1, 2
Affiliation  

The physicochemical properties of a set of 21 different gold nanoparticles (spherical and rod-shaped nanoparticles (NPs) of different diameters with three different surface coatings) were studied. Protein corona formation, in vitro uptake, effect on cell viability and proliferation, and in vivo biodistribution of these NPs were determined. The relation of the results of the different NPs was analyzed by hierarchical cluster analysis, which will tell which NPs have the most similar physicochemical properties and biological effects, without having to specify individual physicochemical parameters. The results show that the physicochemical properties of gold nanoparticles (Au NPs) are mainly accounted for by their hydrodynamic diameter and their zeta-potential. The formation of the protein corona is determined by the pH-dependence of their zeta-potential. While several reports found that in vitro uptake and in vivo biodistribution of NPs are correlated to individual physicochemical parameters, e.g., size, shape, or surface chemistry, such direct dependence in the investigated multidimensional set of NPs was not found in our study. This most likely is due to entanglement of the different parameters, which complicates the prediction of the biological effect of NPs in case multiple physicochemical properties are simultaneously varied. The in vitro uptake and in vivo biodistribution of NPs seem to be not directly driven by the protein corona, but the physicochemical properties determine as well the corona as they influence in vitro/in vivo behaviors, and thus the effect of the protein corona would be rather indirect.

中文翻译:

不同理化性质的纠缠如何使金纳米粒子的体内体外相互作用的预测复杂化

研究了一组21种不同的金纳米颗粒(具有三种不同表面涂层的不同直径的球形和棒状纳米颗粒(NPs))的理化性质。蛋白质电晕的形成,体外摄取,对细胞活力和增殖以及体内的影响确定了这些NP的生物分布。通过分级聚类分析来分析不同NP的结果之间的关系,这将告诉您哪些NP具有最相似的理化性质和生物学效应,而无需指定各个理化参数。结果表明,金纳米颗粒(Au NPs)的理化性质主要由其流体动力学直径和ζ电位决定。蛋白质电晕的形成取决于其Zeta电位的pH依赖性。尽管有几篇报道发现NP的体外吸收和体内生物分布与个体理化参数有关,例如G,大小,形状或表面化学性质,在我们的研究中未发现这种直接依赖于所研究的NP多维集。这很可能是由于不同参数的纠缠所致,在多种理化特性同时变化的情况下,NPs生物学效应的预测变得复杂。在体外摄取和体内NP的生物分布似乎是不直接由蛋白质电晕驱动,但是物理化学性质确定以及电晕,因为它们影响在体外/体内的行为,并因此蛋白质电晕的影响将是相当间接的。
更新日期:2018-09-13
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