A method for simultaneously determining indaziflam and its five metabolites in soil, water, and fruits using ultraperformance liquid chromatography/tandem mass spectrometry was established. The analytes were eluted in <4.5 min. Positive electrospray ionization mode was used. The analytes were extracted using acetonitrile containing 1% ammonium hydroxide, and then the extracts were purified using octadecylsilane and PRiME HLB cartridges. The quantification limits were 0.01–1.01 μg kg^–1. The linearities of the calibrations for all analytes were excellent (R² > 0.9952). The recoveries at spike concentrations of 0.01, 0.1, and 1 mg kg^–1 were 81.3–112.1%. The intraday and interday relative standard deviations were <13.5% and <12.3%, respectively. The method was successfully used to determine indaziflam and its five metabolites in samples from markets and fields. The results confirmed that the method is an effective and robust procedure for routinely determining indaziflam and its metabolites in soil, water, and fruit samples.

中文翻译：

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改良的QuEChERS和UHPLC-MS / MS方法开发并验证土壤，水和水果中的靛蓝及其五种代谢物

建立了一种同时使用超高效液相色谱/串联质谱法同时测定土壤，水和水果中吲达唑火焰及其五种代谢物的方法。在<4.5分钟内洗脱分析物。使用正电喷雾电离模式。使用含有1％氢氧化铵的乙腈提取分析物，然后使用十八烷基硅烷和PRiME HLB柱纯化提取物。定量限为0.01–1.01μgkg ^–1。所有分析物的校准线性都非常好（R ² > 0.9952）。峰值浓度为0.01、0.1和1 mg kg ^–1时的回收率是81.3–112.1％。日内和日间相对标准偏差分别<13.5％和<12.3％。该方法已成功用于测定市场和田间样品中的靛蓝及其五种代谢产物。结果证实，该方法是常规测定土壤，水和水果样品中的靛蓝及其代谢物的有效方法。