Due to autotrophic growing capacity and extremely rich secondary metabolism, plants should be preferred targets of synthetic biology. However, developments in plants usually run below those in other taxonomic groups. In this work we engineered genetic circuits capable of logic YES, OR and AND Boolean computation in plant tissues with a visual output signal. The circuits, which are deployed by means of Agrobacterium tumefaciens, perform with the conditional activity of the MYB transcription factor Rosea1 from Antirrhinum majus inducing the accumulation of anthocyanins, plant endogenous pigments that are directly visible to the naked eye or accurately quantifiable by spectrophotometric analysis. The translational fusion of Rosea1 to several viral proteins, such as potyvirus NIb or fragments thereof, rendered the transcription factor inactive. However, anthocyanin accumulation could be restored by inserting protease cleavage sites between both moieties of the fusion and by coexpressing specific proteases, such as potyvirus nuclear inclusion a protease.

中文翻译：

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使用翻译后遗传控制和视觉输出信号的植物布尔计算

由于自养生长能力和极其丰富的次生代谢，植物应成为合成生物学的首选目标。然而，植物的发展通常低于其他分类群的发展。在这项工作中，我们设计了能够在具有视觉输出信号的植物组织中进行逻辑“是”、“或”和“与”布尔计算的基因电路。这些电路通过根癌农杆菌部署，具有来自金鱼草的 MYB 转录因子 Rosea1 的条件活性诱导肉眼直接可见或通过分光光度分析准确量化的植物内源性色素花青素的积累。Rosea1 与几种病毒蛋白的翻译融合，如马铃薯病毒 NIb 或其片段，使转录因子失活。然而，花青素的积累可以通过在融合的两个部分之间插入蛋白酶切割位点和通过共表达特定的蛋白酶，如马铃薯病毒核内含蛋白酶来恢复。