A novel polysaccharide (MOP-2) was extracted and purified from the leaves of Moringa oleifera by hot water extraction and chromatographic purification. Structural characterization revealed that MOP-2 had an average molecular weight of 155.35 kDa and consisted of arabinose, glucose and galactose with molar percentages of 35.8, 6.67, and 57.53%, respectively. The backbone chain of MOP-2 was proven to be →3,6)-β-D-Galp(1→ and →6)-β-D-Galp-(1→, and the side chains contained →5)-α-L-Araf-(1→, α-L-Araf-(1→, and α-D-Glcp-(1→. In vitro immunomodulatory assay revealed that MOP-2 could significantly enhance the proliferation and pinocytic capacity of the RAW264.7 macrophage cells. Furthermore, MOP-2 promoted the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by activating mRNA expressions of iNOS, IL-6 and TNF-α. These findings suggest that MOP-2 can be developed as a novel natural immunoregulatory agent in functional foods.

从辣木的叶片中提取并通过热水提取和色谱纯化纯化了一种新型多糖（MOP-2）。结构表征表明，MOP-2的平均分子量为155.35 kDa，由阿拉伯糖，葡萄糖和半乳糖组成，摩尔百分比分别为35.8、6.67和57.53％。MOP-2的主链被证明是→3,6）-β-d-Gal的p（1→和→6）-β-d-Gal的对-（1→，和侧链包含→5） -α-L-阿拉˚F - （1→，α-L-阿糖胞苷˚F - （1→，和α-d-GLC p - （1→体外免疫调节试验表明，MOP-2可以显着增强RAW264.7巨噬细胞的增殖和胞吞能力。此外，MOP-2通过激活iNOS，IL的mRNA表达，促进了活性氧（ROS），一氧化氮（NO），白介素6（IL-6）和肿瘤坏死因子-α（TNF-α）的分泌。 -6和TNF-α。这些发现表明，MOP-2可以作为功能性食品中的新型天然免疫调节剂而开发。