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Colorimetric detection of L-histidine based on the target-triggered self-cleavage of swing-structured DNA duplex-induced aggregation of gold nanoparticles
Microchimica Acta ( IF 5.7 ) Pub Date : 2018-09-12 , DOI: 10.1007/s00604-018-2987-z
Yunfei Jiao , Qingyun Liu , Hong Qiang , Zhengbo Chen

AbstractA rapid, highly sensitive and selective colorimetric assay is presented for visually detecting L-histidine. It is based on L-histidine-triggered self-cleavage of DNA duplex-induced gold nanoparticle (AuNP) aggregation. The citrate-capped AuNPs easily aggregate in a high concentration of salt environment. However, in the presence of L-histidine aptamers (DNA1 and DNA2), the partial strands of DNA1 and DNA2 hybridize to form a DNA duplex with a swing structure. The swing-like DNA duplexes are adsorbed on the surface of AuNPs to improve the stability of AuNPs, and the AuNPs also are better dispersed in high-salt media. When L-histidine is added to the solutions, it catalyzes the self-cleavage of DNA1 to form many single-stranded DNA (ssDNA) fragments. These ssDNA segments are adsorbed on the AuNPs and weaken the stability of AuNPs. Hence, the AuNPs aggregate in high-salt environment, and this results in a red-to-blue color change. Under the optimized conditions, L-histidine can be determined with a limit of detection of 3.6 nM. In addition, the sensor was successfully applied to the determination of L-histidine in spiked serum samples. Graphical abstractSchematic of a rapid and homogeneous colorimetric L-histidine assay. It combines L-histidine-triggered self-cleavage of the swing-like DNA duplexes and self-cleavage of DNA-induced AuNP aggregation.

中文翻译:

基于摆动结构 DNA 双链体诱导的金纳米粒子聚集的靶标触发自裂解 L-组氨酸的比色检测

摘要提出了一种快速、高灵敏度和选择性的比色法,用于视觉检测 L-组氨酸。它基于 L-组氨酸触发的 DNA 双链体诱导的金纳米颗粒 (AuNP) 聚集的自切割。柠檬酸盐封端的 AuNPs 很容易在高浓度盐环境中聚集。然而,在 L-组氨酸适体(DNA1 和 DNA2)存在的情况下,DNA1 和 DNA2 的部分链杂交形成具有摆动结构的 DNA 双链体。摆动状DNA双链体吸附在AuNPs表面以提高AuNPs的稳定性,并且AuNPs在高盐介质中也能更好地分散。当 L-组氨酸加入到溶液中时,它会催化 DNA1 的自我切割,形成许多单链 DNA (ssDNA) 片段。这些 ssDNA 片段被吸附在 AuNPs 上并削弱了 AuNPs 的稳定性。因此,AuNPs 在高盐环境中聚集,这导致红色到蓝色的颜色变化。在优化条件下,L-组氨酸的检测限为3.6 nM。此外,该传感器还成功应用于加标血清样品中L-组氨酸的测定。图形摘要快速和均质比色 L-组氨酸测定的示意图。它结合了 L-组氨酸触发的摆动样 DNA 双链体的自切割和 DNA 诱导的 AuNP 聚集的自切割。图形摘要快速和均质比色 L-组氨酸测定的示意图。它结合了 L-组氨酸触发的摆动样 DNA 双链体的自切割和 DNA 诱导的 AuNP 聚集的自切割。图形摘要快速和均质比色 L-组氨酸测定的示意图。它结合了 L-组氨酸触发的摆动样 DNA 双链体的自切割和 DNA 诱导的 AuNP 聚集的自切割。
更新日期:2018-09-12
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