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A dual-mode sensor for colorimetric and fluorescent detection of nitrite in hams based on carbon dots-neutral red system
Meat Science ( IF 7.1 ) Pub Date : 2018-09-09 , DOI: 10.1016/j.meatsci.2018.09.006
Xuetao Hu , Jiyong Shi , Yongqiang Shi , Xiaobo Zou , Haroon Elrasheid Tahir , Mel Holmes , Wen Zhang , Xiaowei Huang , Zhihua Li , Yiwei Xu

Nitrite residue in hams was detected by a fluorescent and colorimetric sensor based on carbon dots (C-dots) and neutral red (NR). C-dots with green fluorescence was synthesized by a microwave-assisted method. This novel sensor was fabricated by C-dots as donors and NR as acceptors. The presence of nitrite led to decrease of absorbance and increase of fluorescence. Colorimetric and fluorescent methods for nitrite detection were developed with excellent correlation coefficients (R2 = 0.995 and 0.991) and low limits of detection (196 nM and 0.518 nM). Moreover, nitrite residue in seven types of ham was detected by the colorimetric and fluorescent methods which were verified by a standard method. The results obtained by the proposed method were comparable and agree with that of the Griess-based method (relative errors<5%). C-dots-NR system as a sensor has a potential application for nitrite detection in hams to monitor its quality and safety.



中文翻译:

基于碳点-中性红系统的双模式传感器,用于比色和荧光检测火腿中的亚硝酸盐

通过基于碳点(C点)和中性红(NR)的荧光和比色传感器检测火腿中的亚硝酸盐残留。通过微波辅助法合成具有绿色荧光的C点。这种新颖的传感器是由C点作为供体,而NR作为受体而制造的。亚硝酸盐的存在导致吸光度降低和荧光增强。开发了具有良好相关系数(R 2的比色法和荧光法用于亚硝酸盐检测的方法。 = 0.995和0.991)和低检测限(196 nM和0.518 nM)。此外,通过比色法和荧光法检测了七种火腿中的亚硝酸盐残留物,并通过标准方法进行了验证。通过所提出的方法获得的结果是可比的,并且与基于Griess的方法(相对误差<5%)相吻合。C-dots-NR系统作为传感器在火腿中亚硝酸盐检测方面具有潜在的应用前景,以监测其质量和安全性。

更新日期:2018-09-09
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