This study examined the impacts of Macondo oil from the Deepwater Horizon oil spill, both alone and in conjunction with exposure to the known fish pathogen Vibrio anguillarum, on the expression of five immune-related gene transcripts of red snapper (il8, il10, tnfa, il1b, and igm). In order to elucidate this impact, six different test conditions were used: one Control group (No oil/No pathogen), one Low oil/No pathogen group (tPAH50 = 0.563 μg/L), one High oil/No pathogen group (tPAH50 = 17.084 μg/L, one No oil/Pathogen group, one Low oil/Pathogen group (tPAH50 = 0.736 μg/L), and one High oil/Pathogen group (tPAH50 = 15.799 μg/L). Fish were exposed to their respective oil concentrations for one week. On day 7 of the experiment, all fish were placed into new tanks (with or without V. anguillarum) for one hour. At three time points (day 8, day 10, and day 17), fish organs were harvested and placed into RNAlater, and qPCR was run for examination of the above specific immune genes as well as cyp1a1. Our results suggest that cyp1a1 transcripts were upregulated in oil-exposed groups throughout the experiment, confirming oil exposure, and that all five immune gene transcripts were upregulated on day 8, but were generally downregulated or showed no differences from controls on days 10 and 17. Finally, both oil and pathogen exposure had impacts on growth.

这项研究研究了深水地平线漏油事件中的Macondo油单独或与已知鱼类病原体鳗弧菌一起暴露对红鲷鱼（il8，il10，tnfa，il1b和igm ）。为了阐明这种影响，使用了六种不同的测试条件：一个对照组（无油/无病原体），一个低油/无病原体组（tPAH50 = 0.563μg/ L），一个高油/无病原体组（tPAH50） = 17.084μg/ L，一个无油/致病菌组，一个低油/致病菌组（tPAH50 = 0.736μg/ L），和一个高油/致病菌组（tPAH50 = 15.799μg/ L）。油浓度为一周，在实验的第7天，将所有鱼放入新的鱼缸（有或没有V. anguillarum）中放置1小时，在三个时间点（第8天，第10天和第17天），将鱼的器官收获并放入RNAlater中，和qPCR是为上述特定的免疫基因的检查运行以及CYP1A1。我们的研究结果表明，CYP1A1 在整个实验过程中，暴露于油的组中的转录本均被上调，从而证实了暴露于油中，并且所有五个免疫基因转录本在第8天均被上调，但通常在10和17天被下调或与对照组无差异。最后，油脂和病原体暴露对生长有影响。