Abstract Magnet-assisted transfection or magnetofection refers to the delivery of nucleic acids to target cells with the help of conjugated superparamagnetic iron oxide nanoparticles (SPIONs) and external magnetic fields generated by permanent magnet plates. The external magnetic field, among other parameters, directly affects the transfection efficiency. However, standard permanent magnet plates generate static magnetic fields which are not as effective compared to time-varying, dynamic fields. In this work, we show a new and novel “AC magnet plate” compatible with standard 96-well cell culture plates, which can be easily adapted for benchtop use in a typical lab setting. We provide full design details, modelling, fabrication, measurement and testing on human embryonic kidney cells (HEK 293) to show transfection improvement. We perform magnetofection under different field conditions and show that with increasing AC content, efficiency of transfection is improved.

中文翻译：

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用于增强氧化铁纳米颗粒共轭核酸磁转染的交替磁场板

摘要 磁体辅助转染或磁转染是指在共轭超顺磁性氧化铁纳米粒子 (SPION) 和永磁板产生的外部磁场的帮助下，将核酸递送至靶细胞。外部磁场等参数直接影响转染效率。然而，标准永磁板产生的静态磁场与时变动态磁场相比并不那么有效。在这项工作中，我们展示了一种与标准 96 孔细胞培养板兼容的新型“交流磁板”，它可以很容易地适应典型实验室环境中的台式使用。我们提供人类胚胎肾细胞 (HEK 293) 的完整设计细节、建模、制造、测量和测试，以显示转染改进。