The remediation and subsequent use of saline-alkaline land are of great significance to ecological environment construction and sustainable agricultural development. Iris halophila Pall. is a salt-tolerant medicinal and ornamental plant, which has good application prospects in the ecological construction of saline-alkaline land; therefore, study of the molecular mechanisms of salt tolerance in I. halophila has important theoretical and practical value. To evaluate the molecular mechanism of the response of I. halophila to salt toxicity, I. halophila seedlings were treated with salt (300 mM NaCl) and subjected to deep RNA sequencing. The clean reads were obtained and assembled into 297,188 unigenes. Among them, 1120 and 100 salt-responsive genes were identified in I. halophila shoots and roots, respectively. Among them, the key flavonoid and lignin biosynthetic genes, hormone signaling genes, sodium/potassium ion transporter genes, and transcription factors were analyzed and summarized. Quantitative reverse-transcription PCR analysis strengthened the reliability of the RNA sequencing results. This work provides an overview of the transcriptomic responses to salt toxicity in I. halophila and identifies the responsive genes that may contribute to its reduced salt toxicity. These results lay an important foundation for further study of the molecular mechanisms of salt tolerance in I. halophila and related species.

盐碱地的修复和后续利用对生态环境建设和农业可持续发展具有重要意义。鸢尾虹膜颇尔。是一种耐盐的药用和观赏植物，在盐碱地的生态建设中具有良好的应用前景。因此，研究嗜盐气单胞菌的耐盐分子机制具有重要的理论和实用价值。为了评估嗜盐杆菌对盐毒性反应的分子机制，嗜盐杆菌幼苗用盐（300 mM NaCl）处理并进行深度RNA测序。获得了干净的读物，并组装成297,188个单基因。其中，在嗜盐气单胞菌的芽和根中分别鉴定出1120和100个盐响应基因。其中，对关键的类黄酮和木质素生物合成基因，激素信号传导基因，钠钾离子转运蛋白基因和转录因子进行了分析和总结。定量逆转录PCR分析增强了RNA测序结果的可靠性。这项工作概述了盐杆菌中盐毒性的转录组反应。并鉴定出可能导致其盐毒性降低的反应性基因。这些结果为进一步研究嗜盐菌和相关物种的耐盐分子机制奠定了重要的基础。