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Characterization of Laguncularia racemosa transcriptome and molecular response to oil pollution
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2018-09-07 , DOI: 10.1016/j.aquatox.2018.09.001
Fernanda Alves de Freitas Guedes , Priscilla de Barros Rossetto , Fábia Guimarães , Maurício Wolf Wilwerth , Jorge Eduardo Santos Paes , Marisa Fabiana Nicolás , Fernanda Reinert , Raquel Silva Peixoto , Márcio Alves-Ferreira

Mangroves are ecosystems of economic and ecological importance. Laguncularia racemosa (Combretaceae), popularly known as white mangrove, is a species that greatly contributes to the community structure of neotropical and West African mangrove forests. Despite the significance of these ecosystems, they have been destroyed by oil spills that can cause yellowing of leaves, increased sensitivity to other stresses and death of trees. However, the molecular response of plants to oil stress is poorly known. In this work, Illumina reads were de novo assembled into 46,944 transcripts of L. racemosa roots and leaves, including putative isoform variants. In addition to improving the genomic information available for mangroves, the L. racemosa assembled transcriptome allowed us to identify reference genes to normalize quantitative real-time PCR (qPCR) expression data from oil-stressed mangrove plants, which were used in RNASeq validation. The analysis of expression changes induced by the oil exposure revealed 310 and 286 responsive transcripts of leaves and roots, respectively, mainly up-regulated. Enriched GO categories related to chloroplasts and photosynthesis were found among both leaf and root oil-responsive transcripts, while “response to heat” and “response to hypoxia” were exclusively enriched in leaves and roots, respectively. The comparison of L. racemosa 12-h-oil-stressed leaf expression profile to previous Arabidopsis heat-stress studies and co-expression evidence also pointed to similarities between the heat and oil responses, in which the HSP-coding genes seem to play a key role. A subset of the L. racemosa oil-responsive root genes exhibited similar up-regulation profiles to their Arabidopsis homologs involved in hypoxia responses, including the HRA1 and LBD41 TF-coding genes. Genes linked to the ethylene pathway such as those coding for ERF TFs were also modulated during the L. racemosa root response to oil stress. Taken together, these results show that oil contamination affects photosynthesis, protein metabolism, hypoxia response and the ethylene pathway in L. racemosa 12-h-oil-exposed leaves and roots.



中文翻译:

紫花苜蓿转录组的表征及对油污的分子响应

红树林是具有经济和生态重要性的生态系统。Laguncularia racemosa(Combretaceae),俗称白红树林,是对新热带和西非红树林的群落结构做出巨大贡献的物种。尽管这些生态系统具有重要意义,但它们仍被溢油破坏,这些溢油可导致叶片发黄,增加对其他压力的敏感性和树木的死亡。然而,人们对植物对油胁迫的分子反应知之甚少。在这项工作中,从头开始将Illumina的阅读读物组装成总状体L. racemosa根和叶的46,944个转录本,包括推定的亚型变体。除了改善可用于红树林的基因组信息外,总状色沙门氏菌(L. racemosa)组装的转录组使我们能够鉴定参考基因,以标准化来自油胁迫的红树林植物的定量实时PCR(qPCR)表达数据,该数据用于RNASeq验证。暴露于油中引起的表达变化的分析表明,叶和根分别具有310和286个响应转录本,主要是上调的。在叶和根油响应性转录物中都发现了与叶绿体和光合作用相关的丰富的GO类别,而“热响应”和“缺氧响应”分别仅在叶和根中富集。的比较L.总状12-H-油强调叶表达谱以前拟南芥热应激研究和共表达证据也指出了热反应和油反应之间的相似性,其中HSP编码基因似乎起着关键作用。沙门氏菌油应答性根基因的一个子集显示出与参与低氧应答的拟南芥同源物相似的上调谱,包括HRA1LBD41 TF编码基因。与乙烯途径相关的基因,例如那些编码ERF TF的基因,也在总油菜根对油胁迫的响应过程中被调节。综上所述,这些结果表明,油污染会影响总状参的光合作用,蛋白质代谢,缺氧反应和乙烯途径 暴露于油中的12小时的叶子和根。

更新日期:2018-09-07
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