In this work, poly(glycidyl methacrylate-ethyleneglycol dimethacrylate) monolith functionalized with copper tetra(N-carbonylacrylic) aminephthalocyanine and iminodiacetic acid was successfully synthesized. Owing to hydrogen bonding and hydrophilic interactions, the monolith exhibited good performance for glycopeptide enrichment. When the tryptic digests of horseradish peroxidase were enriched by the developed monolith, a total of 20 glycopeptides could be captured and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis with a detection limit as low as 0.5 fmol μL⁻¹. With the mixture of bovine serum albumin and horseradish peroxidase digests (200:1, m/m) as the sample, 14 glycopeptides were identified after enrichment, showing the high selectivity of the monolith. Moreover, the functionalized monolith exhibited good stability and reproducibility. It was successfully applied to enrich glycopeptides from human serum, demonstrating its potential applications in selective and efficient capture of glycopeptides in complex biological samples.

中文翻译：

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四（N-羰基丙烯酸）氨基酞菁铜官能化的两性离子聚合物整料的制备，用于糖肽的高特异性捕获

在这项工作中，成功地合成了由四（N-羰基丙烯酸）胺酞菁铜和亚氨基二乙酸官能化的聚（甲基丙烯酸缩水甘油酯-乙二醇二甲基丙烯酸乙二醇酯）整体料。由于氢键和亲水相互作用，整体柱表现出良好的糖肽富集性能。当开发的整体柱富集辣根过氧化物酶的胰蛋白酶消化物时，可以通过基质辅助激光解吸/电离飞行时间质谱分析法捕获和鉴定总共20种糖肽，检测限低至0.5 fmolμL ^-1。与牛血清白蛋白和辣根过氧化物酶消化物的混合物（200：1，m / m）作为样品，富集后鉴定出14种糖肽，显示出整体具有很高的选择性。此外，官能化的整料表现出良好的稳定性和可再现性。它已成功地用于从人血清中富集糖肽，证明了其在选择性和有效捕获复杂生物样品中糖肽方面的潜在应用。