³¹P NMR is a valuable tool to study phosphorus-containing biomolecules from complex mixtures. One important group of such molecules are phosphorus-containing emulsifiers, including lecithins and ammonium phosphatides (AMPs), which are used in chocolate production. By developing extraction protocols and applying high resolution ³¹P nuclear magnetic resonance (NMR), we enable identification of the type of emulsifier used in chocolate. We furthermore demonstrate that this method allows quantification of AMPs in chocolate. To our knowledge, this is the first method that allows verification of the type and amount of emulsifier present in chocolate samples.

中文翻译：

---

使用³¹ P NMR光谱对巧克力中的磷酸铵乳化剂进行定量

³¹ P NMR是研究复杂混合物中含磷生物分子的宝贵工具。这种分子的重要一类是含磷的乳化剂，包括卵磷脂和磷脂铵（AMPs），它们用于巧克力生产。通过开发提取协议并应用高分辨率³¹ P核磁共振（NMR），我们可以识别巧克力中使用的乳化剂类型。我们进一步证明了该方法可以定量巧克力中的AMP。据我们所知，这是第一种可以验证巧克力样品中存在的乳化剂类型和数量的方法。