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Importance of TLR9-IL23-IL17 axis in inflammatory bowel disease development: Gene expression profiling study
Clinical Immunology ( IF 8.6 ) Pub Date : 2018-09-05 , DOI: 10.1016/j.clim.2018.09.001
Sanja Dragasevic , Biljana Stankovic , Aleksandra Sokic-Milutinovic , Tomica Milosavljevic , Tamara Milovanovic , Snezana Lukic , Sanja Srzentic Drazilov , Kristel Klaassen , Nikola Kotur , Sonja Pavlovic , Dragan Popovic

Background and aims

Mucosal gene expression have not been fully enlightened in inflammatory bowel disease (IBD). Aim of this study was to define IL23A, IL17A, IL17F and TLR9 expression in different IBD phenotypes.

Methods

Evaluation of mRNA levels was performed in paired non-inflamed and inflamed mucosal biopsies of newly diagnosed 50 Crohn's disease (CD) and 54 ulcerative colitis (UC) patients by quantitative real-time PCR analysis.

Results

IL17A and IL17F expression levels were significantly increased in inflamed IBD mucosa. Inflamed CD ileal and UC mucosa showed increased IL23A, while only inflamed CD ileal samples showed increased TLR9 mRNA level. Correlation between analysed mRNAs levels and endoscopic and clinical disease activity were found in UC, but only with clinical activity in CD.

Conclusion

Both CD and UC presented expression of Th17-associated genes. Nevertheless, expression profiles between different disease forms varies which should be taken into account for future research and therapeutics strategies.



中文翻译:

TLR9-IL23-IL17轴在炎症性肠病发展中的重要性:基因表达谱研究

背景和目标

在炎症性肠病(IBD)中,粘膜基因表达尚未得到充分的启发。这项研究的目的是定义不同IBD表型中的IL23A,IL17A,IL17FTLR9表达。

方法

通过定量实时PCR分析,对新诊断的50例克罗恩病(CD)和54例溃疡性结肠炎(UC)患者的成对非发炎和发炎的黏膜活检组织中的mRNA水平进行了评估。

结果

在发炎的IBD粘膜中IL17AIL17F表达水平显着增加。CD回肠和UC黏膜发炎显示IL23A升高,而CD回肠样本发炎的TLR9 mRNA水平升高。在UC中发现分析的mRNA水平与内窥镜和临床疾病活动之间的相关性,但仅与CD中的临床活动相关。

结论

CD和UC均显示Th17相关基因的表达。然而,不同疾病形式之间的表达谱是不同的,在将来的研究和治疗策略中应予以考虑。

更新日期:2018-09-05
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