Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21. More importantly, the target microRNA-21 could be regenerated with ATP as the fuel rather than a nucleotide segment in conventional approaches, which made the proposed strategy simple and efficient due to the high affinity and strength of the aptamer–target interaction.

中文翻译：

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ATP激发的核酸信号扩增策略可实现高度灵敏的microRNA检测†

在本文中，提出了基于脚趾介导的链置换（TMSD）和荧光共振能量转移（FRET）的以三磷酸腺苷（ATP）为燃料的核酸信号放大策略，用于microRNA-21的高灵敏度检测。更重要的是，在常规方法中，目标microRNA-21可以用ATP作为燃料而不是核苷酸片段进行再生，这归因于适体与靶标相互作用的高亲和力和强度，使拟议的策略简单有效。