Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.

中文翻译：

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光合作用底盘的诞生：一个使微藻衣藻（Chlamydomonas reinhardtii）中具有合成生物学功能的MoClo工具包

微藻被认为是有前途的生物，可以根据其光合能力开发创新概念，比异养宿主提供更多可持续的生产。然而，要实现其作为绿色细胞工厂的潜力，主要的挑战是使微藻更易于工程设计。快速且可预测的基因操作的一种有前途的方法是使用标准化的合成生物学工具和工作流程。为此，我们为绿色微藻衣藻（Chlamydomonas reinhardtii）开发了模块化克隆工具包。。它基于采用标准语法的金门克隆，包括119个公开分布的遗传部分，其中大部分已在几种菌株中进行了功能验证。它包含启动子，UTR，终止子，标签，报告基因，抗生素抗性基因和内含子，这些克隆在各个位置，以实现最大的模块化。该工具包可快速构建用于基础研究和藻类生物技术的工程细胞。这项工作将使衣藻成为可持续合成生物学的下一个底盘。