Recently, interleukin‐5 receptor alpha (IL‐5RA) was reported to be a key surface molecule in the regulation of eosinophil proliferation. In this study, a highly sensitive aptamer for IL‐5RA was applied to an electrochemical biosensor. The rod‐shaped gold electrodes with circular gold surface were used. The aptasensor specifically detected membrane‐bound IL‐5RA on human eosinophils according to the number of the eosinophils. We further examined the usability of soluble IL‐5RA (sIL‐5RA) in the serum as a novel biomarker for the diagnosis of eosinophil‐related diseases. When eosinophilic inflammation occurs, the concentration of sIL‐5RA in the blood is highly elevated. To diagnose eosinophilic inflammation, the aptasensor was calibrated in phosphate‐buffered saline (PBS) to detect sIL‐5RA. The detection limit of the aptasensor in PBS was 1.69 pg/mL, which is lower than that of commercially available ELISA kits. The dynamic range of the aptasensor was 10–10,000 pg/mL, with high specificity over other serum proteins and interleukin family members. The aptasensor was capable of distinguishing between patients with eosinophilic asthma (EA) and normal controls (NCs) based on serum sIL‐5RA levels. Consequently, the fabricated aptasensor will contribute to the development of novel diagnostic systems for IL‐5RA‐related diseases.

中文翻译：

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使用可溶白细胞介素-5受体α的无标记阻抗自适应传感器的新型嗜酸性粒细胞诊断。

最近，据报道白细胞介素-5受体α（IL-5RA）是调节嗜酸性粒细胞增殖的关键表面分子。在这项研究中，将用于IL-5RA的高度敏感的适体应用于电化学生物传感器。使用具有圆形金表面的棒状金电极。aptasensor根据嗜酸性粒细胞的数量特异性检测人嗜酸性粒细胞的膜结合IL-5RA。我们进一步检查了血清中可溶性IL-5RA（sIL-5RA）作为诊断嗜酸性粒细胞相关疾病的新型生物标志物的可用性。当发生嗜酸性炎症时，血液中的sIL-5RA浓度会大大升高。为了诊断嗜酸性粒细胞炎症，在磷酸缓冲盐溶液（PBS）中对aptasensor进行了校准，以检测sIL-5RA。PBS中适体传感器的检出限为1.69 pg / mL，低于市售ELISA试剂盒。适体传感器的动态范围为10–10,000 pg / mL，对其他血清蛋白和白介素家族成员具有高度特异性。aptasensor能够根据血清sIL-5RA水平区分嗜酸性粒细胞性哮喘（EA）和正常对照组（NCs）。因此，制造的适体传感器将有助于开发IL-5RA相关疾病的新型诊断系统。