In order to monitor the pollution of polycyclic aromatic hydrocarbons (PAHs) in the seawater environment, screening biomarkers capable of monitoring PAHs is the focus of many studies. The transcriptomic profiles of the digestive gland tissue from the R. philippinarum groups after the exposure to BaP (4 μg/L) at four time points (0, 0.5, 6 and 15 days) were investigated to globally screen the key genes and pathways involved in the responses to short-term stress and long-term adaptation of BaP resistance. By comparative transcriptome analysis, 233, 282 and 58 differentially expressed genes (DEGs) were identified at 0.5 day, 6 day and 15 day (vs 0 day). The differential expression genes were related to stress response, detoxification metabolic process and innate immunity. DEGs of each group at different stages were clustered in six profiles based on gene expression pattern. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis were used on all genes to determine the biological functions and processes. We selected Multidrug resistance protein 3 (MRP3), transcriptional regulator ATRX-like isoform X2 (ATRX) as biomarker indicator genes for short-term pollution monitoring and NADH dehydrogenase [ubiquinone] 1 (NQO1), Complement C1q-like protein 4 (C1q), Glutathione-S-transferase theta (GST), E3 ubiquitin-protein ligase (E3) for long-term pollution monitoring based on the different expression patterns and the function in detoxification and antioxidant defense system. Besides, the expression of seven genes was measured through Quantitative real-time PCR (qPCR) according to their gene expression patterns which was confirmed by the DGE analysis. Taken together, adoption of transcriptomic analysis to explore the bivalves’ mRNA abundance changes and detoxification metabolic mechanism under the BaP stress at different time points can aid the development of sensitive and informed molecular endpoints for application towards ecotoxicogenomic monitoring of bivalves.

为了监测海水环境中多环芳烃（PAHs）的污染，筛选能够监测PAHs的生物标记物是许多研究的重点。菲律宾蛤仔消化腺组织的转录组谱在四个时间点（0、0.5、6和15天）暴露于BaP（4μg/ L）后的两组进行调查，以全面筛选与短期压力和长期适应反应有关的关键基因和途径BaP抵抗力。通过比较转录组分析，分别在0.5天，6天和15天（vs 0天）鉴定出233、282和58个差异表达基因（DEG）。差异表达基因与应激反应，排毒代谢过程和先天免疫有关。根据基因表达模式，将每组处于不同阶段的DEG聚类为六个分布图。对所有基因都使用了基因本体论（GO）和《京都议定书基因与基因组百科全书》（KEGG）通路分析，以确定其生物学功能和过程。我们选择了多药耐药蛋白3（MRP3），转录调节因子ATRX样亚型X2（ATRX）作为生物标志物指示基因，用于短期污染监测和NADH脱氢酶[泛醌] 1（NQO1），补体C1q样蛋白4（C1q），谷胱甘肽-S-转移酶theta（GST） ，E3泛素蛋白连接酶（E3）用于基于不同的表达方式以及在排毒和抗氧化防御系统中的功能进行的长期污染监测。此外，根据DGE分析所证实的基因表达模式，通过定量实时PCR（qPCR）测量了七个基因的表达。在一起