Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia. However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation. In our study, the use of PGC chromatography–ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3). Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries. Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation. Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues. STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.

中文翻译：

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链脲佐菌素诱导的高血糖对小鼠卵巢N和O联蛋白糖基化的影响

蛋白质的翻译后修饰即糖基化会影响细胞行为，结构特性和相互作用，包括在卵巢卵泡发育和闭锁过程中。然而，尽管糖尿病对成功的胚胎植入结果有众所周知的影响，但对卵巢组织中糖尿病中发生的蛋白质糖基化变化知之甚少。在我们的研究中，在负离子模式下使用PGC色谱-ESI质谱能够鉴定链脲佐菌素诱导的（STZ）糖尿病小鼠卵巢组织蛋白上的138 N-聚糖和6 O-聚糖（n = 3）。糖尿病小鼠卵巢唾液酸化，岩藻糖基化和相对较小程度的分支N相对减少与非糖尿病小鼠卵巢相比，其连接的聚糖结构以及其蛋白质上的低聚甘露糖结构的增加。N-聚糖的变化发生在糖尿病肝组织中，但在同一只小鼠的糖尿病卵巢组织中更明显，表明糖尿病对蛋白质糖基化的器官特异性作用。尽管小鼠卵巢的O- GalNAc聚糖含量非常低，但其核心类型为1型和2型。NeuGc：NeuAc比值的相对增加是对照和糖尿病卵巢组织之间的最显着差异。经STZ处理的小鼠还表现出TNF-α和IL1-B炎性细胞因子增加的趋势，这些因子先前已被证明会影响蛋白质糖基化。