Abstract An enzyme based nanocomposite host matrix comprising of Poly(3,4-ethylenedioxythiophene) and 1-Butyl-3-methylimidazolium trifluoromethanesulfonate based ionic liquid functionalized reduced graphene oxide (PEDOT/ILRGO) has been designed for the electrochemical detection of organophosphorus pesticides (OPs). Interactions between reduced graphene oxide and ionic liquid have resulted in better loading of the same onto the PEDOT matrix. A detail redox analysis highlights the increased surface area and more number of charge carriers enabling the redox inhibition mechanism more efficient in the designed electrode. The biosensor works on the principle of generation of thiocholine by reaction between acetylcholinesterase (AChE) and substrate acethylthiocholine iodide (ATChI), which undergoes oxidation resulting in redox peaks. Under the optimized conditions, three different OPs chlorpyrifos (CP), malathion (ML) and methyl parathion (MP) were analyzed by varying concentrations with limit of detection calculated to be 0.04 ng ml−1, 0.117 ng ml−1 and 0.108 ng ml−1 respectively, all below 0.2 µg ml−1 concentration which is their maximum residual limit, hence exhibiting good sensitivity. The prepared sensor offers 91.7% of reactivation and good stability for 15–20 days with 95.7% of initial current response retainment, reflecting its excellent potency as an organophosphorus pesticide sensor.

中文翻译：

---

离子液体辅助聚（3,4-乙撑二氧噻吩）/还原氧化石墨烯基电极的生长：提高了检测饮料中有机磷农药的电催化性能

摘要 设计了一种基于酶的纳米复合基质，该基质包含聚（3,4-亚乙基二氧噻吩）和 1-丁基-3-甲基咪唑三氟甲磺酸盐基离子液体功能化还原氧化石墨烯（PEDOT/ILRGO），用于有机磷农药（OPs）的电化学检测。 ）。还原氧化石墨烯和离子液体之间的相互作用导致其更好地负载到 PEDOT 基质上。详细的氧化还原分析突出显示了增加的表面积和更多数量的电荷载流子，使设计的电极中的氧化还原抑制机制更有效。该生物传感器的工作原理是通过乙酰胆碱酯酶 (AChE) 和底物乙酰基硫胆碱碘化物 (ATChI) 之间的反应生成硫代胆碱，经过氧化产生氧化还原峰。在优化条件下，对三种不同的有机磷毒死蜱 (CP)、马拉硫磷 (ML) 和甲基对硫磷 (MP) 进行了不同浓度的分析，计算出的检测限分别为 0.04 ng ml-1、0.117 ng ml-1 和 0.108 ng ml -1 分别低于 0.2 µg ml-1 浓度，这是它们的最大残留极限，因此表现出良好的灵敏度。制备的传感器提供 91.7% 的再活化和 15-20 天的良好稳定性和 95.7% 的初始电流响应保留，反映了其作为有机磷农药传感器的优异效能。2 µg ml-1 浓度是它们的最大残留限度，因此表现出良好的灵敏度。制备的传感器提供 91.7% 的再活化和 15-20 天的良好稳定性和 95.7% 的初始电流响应保留，反映了其作为有机磷农药传感器的优异效能。2 µg ml-1 浓度是它们的最大残留限度，因此表现出良好的灵敏度。制备的传感器提供 91.7% 的再活化和 15-20 天的良好稳定性和 95.7% 的初始电流响应保留，反映了其作为有机磷农药传感器的优异效能。