Improving the activity and stability of enzymes is significant in enzyme immobilization. Here a facile approach to prepare ring‐like ZIF‐8 colloidosomes and spherical catalase‐embedded ZIF‐8 colloidosomes is developed via one‐step emulsion‐based technique at the water/butanol interface. The influence of the concentrations of ZIF‐8 nanocrystals and Pluronic F127 as well as the oil‐water ratio was investigated. Compared with in situ biomineralization, the colloidosomes prepared via the pickering emulsion method show successful encapsulation of positively charged enzymes. By using catalase as an immobilized model, the immobilized catalase exhibits high biocatalytic activity, stability and recyclability compared with free catalase.

中文翻译：

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基于乳液的酶包埋金属有机骨架胶体

提高酶的活性和稳定性对于酶的固定化具有重要意义。在这里，通过基于水/丁醇界面的一步乳液技术，开发了一种制备环状 ZIF-8 胶体和嵌入球形过氧化氢酶的 ZIF-8 胶体的简便方法。研究了 ZIF-8 纳米晶体和 Pluronic F127 的浓度以及油水比的影响。与原位生物矿化相比，通过皮克林乳液法制备的胶体显示出成功包封带正电荷的酶。以过氧化氢酶为固定化模型，与游离过氧化氢酶相比，固定化过氧化氢酶具有较高的生物催化活性、稳定性和可回收性。