In this study, the effects of a chokeberry fruit crude extract (CE), its ethyl acetate fractions (EAF7 & EAF2) and a polyphenol-rich fraction (PRF) on esterase and lipolytic activities of pancreatic lipase (PL) were investigated. Ethyl acetate fractions, rich in flavonols, phenolic acids and low molecular procyanidins, showed a week inhibitory activity against PL while the CE and PRF, which contained the highly polymerized procyanidins exhibited the highest anti-lipase activity. The kinetic study showed that the inhibitory mode of CE was noncompetitive while PRF behaved like the mixed type inhibitor. Cumulative effects were observed when orlistat was used with either CE or PRF. These results indicate that black chokeberry fruits are a source of PL inhibitors with proanthocyanidins as the most active compounds.

在这项研究中，研究了苦莓果实粗提取物（CE），其乙酸乙酯馏分（EAF7和EAF2）和富含多酚的馏分（PRF）对胰腺脂肪酶（PL）的酯酶和脂解活性的影响。富含黄酮醇，酚酸和低分子原花青素的乙酸乙酯级分显示出对PL的抑制活性，而包含高度聚合的原花青素的CE和PRF则显示出最高的抗脂肪酶活性。动力学研究表明，CE的抑制模式是非竞争性的，而PRF的行为类似于混合型抑制剂。当奥利司他与CE或PRF一起使用时，观察到累积效果。这些结果表明，黑苦莓果实是PL抑制剂的来源，原花青素是活性最高的化合物。