A green synthesis of biocompatible magnetite (Fe₃O₄) nanoparticles (MNPs) using a combination of urea (U) and gram-bean extract (GBE, Cicer arietinum L.) is reported. The particle size of ∼13 nm and highly stable magnetite phase is observed for GBE-U mediated MNPs. On the other hand, the MNPs synthesized using either U or GBE shows larger particle size and uneven size distribution. Interestingly, the sample with particle size ∼13 nm shows optimum heat generation capacity (measured in specific absorption rate, i.e., SAR) near to the therapeutic temperature (43 °C) with least-variance. To investigate the influence of various factors such as variation in MNPs weight concentration (W_t), applied alternating magnetic field (AMF), saturation magnetization (M_s), magnetization rate (R_m), etc. on SAR, a multiple linear regression model (MLRM) is used. The study reveals a positive correlation of SAR with R_m and AMF values while the negative correlation with M_s and W_t. Ultimately, the present green synthesis is the affordable approach for preparing stable and tiny MNPs. Moreover, MLRM is found to be a useful theoretical tool for understanding the influence of MNPs on hyperthermia performance.

报道了使用尿素（U）和豆角豆提取物（GBE，Acerinum L.）的绿色合成生物相容性磁铁矿（Fe ₃ O ₄）纳米颗粒（MNP）。对于GBE-U介导的MNP，观察到约13 nm的粒径和高度稳定的磁铁矿相。另一方面，使用U或GBE合成的MNP显示较大的粒径和不均匀的粒径分布。有趣的是，粒径约为13 nm的样品在接近治疗温度（43°C）时具有最小的变化，显示出最佳的生热能力（以比吸收率（即SAR）衡量）。研究各种因素的影响，例如MNPs重量浓度的变化（W _t），SAR上施加的交变磁场（AMF），饱和磁化强度（M _s），磁化速率（R _m）等，使用多元线性回归模型（MLRM）。研究发现SAR与R _m和AMF值呈正相关，而与M _s和W _t呈负相关。最终，目前的绿色合成方法是制备稳定且微小的MNP的可负担的方法。此外，发现MLRM是了解MNP对热疗性能的影响的有用理论工具。