Abstract A simple and rapid magnetic covalent organic frameworks based on magnetic solid phase extraction method was proposed for effective determination of six steroidal and phenolic endocrine disrupting chemicals in meat samples. Fluorescence labeling combined with high performance liquid chromatography fluorescence detection was used to quantify endocrine disrupting chemicals. Covalent organic frameworks could be a wonderful choice as sorbent for endocrine disrupting chemicals enrichment due to its characteristics of large specific surface area, great structure stability and functional properties. In this work, COF(TpBD) was chosen as functionalized groups to be grafted onto the surface of Fe3O4 microspheres via one-step surface fabrication strategy. The parameters affecting the extraction efficiency were investigated in detail. The adsorption equilibrium was achieved within 5 min. Under the optimized conditions, the obtained Fe3O4@COF(TpBD) materials showed excellent extraction performance for endocrine disrupting chemicals with high sensitivity (1.4–8.7 μg L−1 for LODs and 4.6–29.0 μg L−1 for LOQs) and good linearity (R ≥ 0.9986). Determination of endocrine disrupting chemicals in meats was achieved using the proposed method. Furthermore, the method, validated on spiked meats (including chicken, pork and shrimp), showed satisfactory accuracy, with average recoveries in the 89.6–108.9% range and relative standard deviations ≤6.3%. Additionally, two kinetic models were investigated to recognize the target analytes adsorption mechanism onto Fe3O4@COF(TpBD) materials, and the results indicated that the pseudo-second-order model was the most suitable in describing the adsorption kinetics of endocrine disrupting chemicals on the Fe3O4@COF(TpBD) materials. The proposed method was proved to be feasible for the determination of endocrine disrupting chemicals in complex food samples.

中文翻译：

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基于磁性固相萃取的磁性共价有机骨架用于测定食品样品中的六种甾体和酚类内分泌干扰物

摘要 提出了一种基于磁性固相萃取方法的简单、快速的磁性共价有机骨架，用于有效测定肉类样品中的六种甾体和酚类内分泌干扰物。荧光标记结合高效液相色谱荧光检测用于定量内分泌干扰化学物质。共价有机骨架由于其比表面积大、结构稳定性好和功能特性好等特点，可能成为内分泌干扰物富集吸附剂的绝佳选择。在这项工作中，COF(TpBD) 被选为官能团，通过一步表面制备策略接枝到 Fe3O4 微球表面。详细研究了影响提取效率的参数。在 5 分钟内达到吸附平衡。在优化条件下，获得的 Fe3O4@COF(TpBD) 材料对内分泌干扰物表现出优异的提取性能，具有高灵敏度（LOD 为 1.4-8.7 μg L-1，LOQ 为 4.6-29.0 μg L-1）和良好的线性（ R≥0.9986）。使用所提出的方法实现了肉类中内分泌干扰化学物质的测定。此外，该方法在加标肉类（包括鸡肉、猪肉和虾）上进行了验证，显示出令人满意的准确度，平均回收率在 89.6-108.9% 范围内，相对标准偏差≤6.3%。此外，研究了两种动力学模型以识别目标分析物在 Fe3O4@COF(TpBD) 材料上的吸附机制，结果表明，拟二级模型最适合描述内分泌干扰物质在 Fe3O4@COF(TpBD) 材料上的吸附动力学。该方法被证明适用于复杂食品样品中内分泌干扰物的测定。