Glycosylation is the most common post-translational modification of serum proteins, and changes in the type and abundance of glycans in human serum have been correlated with a growing number of human diseases. While the glycosylation pattern of human serum is well studied, little is known about the profiles of other mammalian species. Here, we report detailed glycosylation profiling of canine serum by hydrophilic interaction chromatography-ultraperformance liquid chromatography (HILIC-UPLC) and mass spectrometry. The domestic dog (Canis familiaris) is a widely used model organism and of considerable interest for a large veterinary community. We found significant differences in the serum N-glycosylation profile of dogs compared to that of humans, such as a lower abundance of galactosylated and sialylated glycans. We also compare the N-glycan profile of canine serum to that of canine IgG – the most abundant serum glycoprotein. Our data will serve as a baseline reference for future studies when performing serum analyses of various health and disease states in dogs.

中文翻译：

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狗血清糖基化分析揭示了与人血清相比的差异

糖基化是血清蛋白最常见的翻译后修饰，人类血清中聚糖类型和丰度的变化与越来越多的人类疾病相关。尽管对人血清的糖基化模式进行了充分的研究，但对其他哺乳动物物种的概况知之甚少。在这里，我们报告通过亲水相互作用色谱-超高效液相色谱（HILIC-UPLC）和质谱分析犬血清的详细糖基化谱。家犬（犬似犬）是一种广泛使用的模型生物，对大型兽医界具有极大的兴趣。我们发现与人相比，狗的血清N-糖基化分布存在显着差异，例如较低的半乳糖基化和唾液酸化聚糖含量。我们还比较了犬血清与犬IgG（最丰富的血清糖蛋白）的N-聚糖谱。当对狗的各种健康和疾病状态进行血清分析时，我们的数据将作为将来研究的基线参考。