The preventive effects of TQS169 peptide on osteoporosis were investigated and the mechanisms of promoting osteogenic differentiation and calcium absorption were also studied in vitro and vivo in this research. Wistar rats were given a low-Calcium diet treated with different doses of peptide TQS169 and extra CaCl₂ for 4 weeks. The content of Calcium, OPG in serum and the Ca content of the femur were increased in high-dose-treated rats. The osteoporosis model induced by retinoic acid was established to measure the related factors. High dosage of TQS169 treated group showed the protective effects. Osteoblastogenesis differentiation of MC3T3-E1 cells treated with TQS169 were accelerated through improving ALP activity, promoting mineral matrix formation and the expression of typical genes of RUNX2, OCN and COL I. Taking all the experiments together, our study confirms the protective effects of TQS169 on osteoporosis and explains the potential mechanism for the first time.

研究了TQS169肽对骨质疏松的预防作用，并在体内和体外研究了促进成骨细胞分化和钙吸收的机制。给予Wistar大鼠低钙饮食，用不同剂量的肽TQS169和额外的CaCl _2处理持续4周。高剂量处理的大鼠血清钙，OPG含量和股骨钙含量增加。建立了视黄酸诱导的骨质疏松模型，以测定相关因素。高剂量的TQS169治疗组具有保护作用。通过改善ALP活性，促进矿物质基质形成以及RUNX2，OCN和COL I的典型基因的表达，可以加速TQS169处理的MC3T3-E1细胞的成骨细胞分化。综合所有实验，我们的研究证实了TQS169对TQS169的保护作用。并首次解释了骨质疏松的潜在机制。