Development of biocompatible polymeric systems capable of cell adhesion and proliferation is a challenging task. Proper cross-linking of small cell adhesive peptide sequences is useful in this respect as it provides the inherent nontoxic environment as well as the cross-linked polymeric network to the cells for adhesion and proliferation. A multiple cross-linking strategy is applied to create a peptide-based cross-linked polymer. Covalent linkage through disulfide bond formation, supramolecular linkage using homoternary complexation by CB[8], and enzymatic cross-linking by HRP-mediated dimerization of tyrosine are used to prepare the cross-linked, peptide-based polymer decorated with cell-adhesive RGDS sequence. The supramolecular cross-linking via CB[8] provided stability as well as brings the RGDS sequences at the surface of the polymer particles. The order of cross-linking allowed to fine-tune the particle size of the polymer and polymer particles of wide range (200–1000 nm) can be prepared by varying the order. The cross-linked polymer particles (P1 and P2) were found to be stable at wide range of temperature and pH. Moreover, as intended, the polymer was noncytotoxic in nature and showed efficient cell adhesion and proliferation property, which can be used for further biological applications.

中文翻译：

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小肽的多次交联以形成具有有效细胞粘附和增殖特性的尺寸可调的生物聚合物

能够细胞粘附和增殖的生物相容性聚合物系统的开发是一项艰巨的任务。小细胞粘附肽序列的正确交联在这方面是有用的，因为它提供了固有的无毒环境以及与细胞的交联聚合物网络以用于粘附和增殖。应用多重交联策略来创建基于肽的交联聚合物。通过形成二硫键的共价键，使用CB的三元络合物通过超分子键[8]以及通过HRP介导的酪氨酸二聚化进行酶交联，来制备以细胞粘附RGDS序列修饰的基于肽的交联聚合物。 。通过CB [8]的超分子交联提供了稳定性，并使RGDS序列进入了聚合物颗粒的表面。可以精细调整聚合物粒径的交联顺序，可以通过改变顺序来制备宽范围（200–1000 nm）的聚合物颗粒。交联的聚合物颗粒（发现P1和P2）在宽的温度和pH范围内是稳定的。此外，按预期，该聚合物本质上是无细胞毒性的，并显示出有效的细胞粘附和增殖特性，可用于进一步的生物学应用。