Modulation of support wettability used for microarray format biosensing has led to an improvement of results. Hydrophobicity of glass chips was set by derivatizing with single vinyl organosilanes of different chain length and silane mixtures. Thiol-ene photochemical linking has been used as effective chemistry for covalent anchoring of thiolated probes. Lowest unspecific binding and highest signal intensity and SNR were obtained with large hydrocarbon chain (C₂₂) silanes or a shorter one (C₁₀) containing fluorine atoms. SNR resulting values are improved, reaching levels higher than 1500 in some cases, when using vinyl silanes modified with 1% C₁₀ alkyl fluorinated one, because mild hydrophobicity was achieved (water contact angle ca. 110°) for all silanes, including the short C₂ and C₃, thus giving rise to smaller and better defined array spots. In addition, unspecific binding of reagents and targets was totally withdrawn. Hence, good-performing surfaces for biosensing applications can be built using appropriate organosilane reagent selection, including fluorinated ones.

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用于微阵列格式生物传感的支持物润湿性的调节导致结果的改善。通过用不同链长的单一乙烯基有机硅烷和硅烷混合物衍生化来设置玻璃碎片的疏水性。硫醇-烯光化学键合已被用作共价锚定硫醇化探针的有效化学方法。使用较大的烃链（C ₂₂）硅烷或较短的含氟原子的硅烷（C ₁₀），可获得最低的非特异性结合以及最高的信号强度和SNR 。使用1％C ₁₀改性的乙烯基硅烷时，SNR结果值得到改善，在某些情况下达到1500以上的水平烷基氟化的一种，因为所有硅烷（包括短的C ₂和C ₃）均实现了适度的疏水性（水接触角约为110°），因此产生了更小，定义更好的阵列点。另外，完全撤消了试剂与靶标的非特异性结合。因此，可以通过选择适当的有机硅烷试剂（包括氟化的有机硅烷试剂）来构建用于生物传感应用的性能良好的表面。

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