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Fluorographene and Graphane as an Excellent Platform for Enzyme Biocatalysis
Chemistry - A European Journal ( IF 4.3 ) Pub Date : 2018-10-18 , DOI: 10.1002/chem.201803397
Soňa Hermanová 1 , Daniel Bouša 2 , Vlastimil Mazánek 2 , David Sedmidubský 2 , Jan Plutnar 2, 3 , Martin Pumera 2 , Zdeněk Sofer 2
Affiliation  

The application of enzymes is a crucial issue for current biotechnological application in pharmaceutical, as well as food and cosmetic industry. Effective platforms for enzyme immobilization are necessary for their industrial use in various biosynthesis procedures. Such platforms must provide high yield of immobilization and retain high activity at various conditions for their large‐scale applications. Graphene derivatives such as hydrogenated graphene (graphane) and fluorographene can be applied for enzyme immobilization with close to 100 % yield that can result to activities of the composites significantly exceeding activity of free enzymes. The hydrophobic properties of graphene stoichiometric derivatives allowed for excellent non‐covalent bonding of enzymes and their use in various organic solvents. The immobilized enzymes retain their high activities even at elevated temperatures. These findings show excellent application potential of enzyme biocatalysts immobilized on graphene stoichiometric derivatives.

中文翻译:

石墨烯和石墨烷是酶生物催化的绝佳平台

酶的应用是当前生物技术在制药,食品和化妆品工业中的关键问题。有效的酶固定平台对于它们在各种生物合成程序中的工业应用是必不可少的。这样的平台必须提供高固定化率,并在各种条件下保持高活性,以进行大规模应用。可以将石墨烯衍生物(例如氢化石墨烯(石墨烷)和氟石墨烯)以接近100%的产率用于酶固定,这可能导致复合材料的活性大大超过游离酶的活性。石墨烯化学计量衍生物的疏水特性使酶具有出色的非共价键结合能力,并可以在各种有机溶剂中使用。固定的酶即使在升高的温度下也保持其高活性。这些发现显示了固定在石墨烯化学计量衍生物上的酶生物催化剂的极好的应用潜力。
更新日期:2018-10-18
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