A new ceramide analog, 1, containing two fluorescent dyes, NBD in the N-acyl part and KFL5 in the alkyl part, was synthesized. The fluorescence from both NBD and KFL5 was detected in living cells in a time-dependent manner. A multi-wavelength fluorescence detector was used to detect ceramide metabolites including sphingosine, sphingosine-1-phosphate, glucosylceramide, and sphingomyelin, which are connected to the fluorescent dyes, simultaneously in a single TLC plate.

中文翻译：

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新型双标记荧光神经酰胺探针的开发，用于监测活细胞中鞘脂的代谢

合成了一种新的神经酰胺类似物1，其中包含两种荧光染料，N酰基部分为NBD，烷基部分为KFL5。NBD和KFL5的荧光都以时间依赖的方式在活细胞中被检测到。使用多波长荧光检测器在单个TLC板中同时检测与荧光染料相连的神经酰胺代谢物，包括鞘氨醇，鞘氨醇1-磷酸，葡萄糖基神经酰胺和鞘磷脂。