A point-of-care diagnostic kit was developed for detection of Morganella morganii using optimized loop-mediated isothermal amplification (LAMP) technique within less than an hour. In that regard, dimethyl sulfoxide (DMSO) was utilized together with betaine and all variables were optimized to improve the efficiency of the method. Moreover, surface presentation of antigens protein was targeted and six unique primers were designed. Endpoint turbidity analysis was performed at 550 nm to measure the tetravalent anion (pyrophosphate) released during the reaction. The specificity of the method was evaluated using nine closely related bacterial species as well as its sensitivity. It was shown that the improved LAMP assay could significantly distinguish M. morganii from other bacteria while the sensitivity was determined to be 0.2 CFU mL^-1.

开发了一种即时诊断工具包，用于在不到一个小时的时间内使用优化的环介导的等温扩增（LAMP）技术检测摩根氏摩根氏菌。在这方面，将二甲基亚砜（DMSO）与甜菜碱一起使用，并对所有变量进行了优化，以提高该方法的效率。此外，靶向抗原蛋白的表面呈递，并设计了六种独特的引物。在550 nm进行终点浊度分析，以测量反应过程中释放的四价阴离子（焦磷酸）。使用九种密切相关的细菌种类及其灵敏度评估了该方法的特异性。结果表明，改进的LAMP测定法可以明显地区分摩根氏菌。同时测定其他细菌的敏感性为0.2 CFU mL ^-1。