Nucleoside-adduct analysis by liquid chromatography mass spectrometry is a powerful tool in genotoxicity studies. Efforts to date have quantified an impressive array of DNA damage products, although methodological diversity suggests quantification is still a challenging task. For example, inadequate co-examination of normal nucleosides, cumbersome sample preparation and large DNA requirements were identified to be recurring issues. A six-minute ultra-performance liquid chromatography method is presented which adequately separates seven candidate nucleoside-adducts from the four unmodified nucleosides. The method was sensitive to 1 adduct per 10⁸ normal bases with 20 µg DNA input for most targets. The method was shown to be accurate (81–119% across quintuplets of six tissue types) and precise (relative standard deviation 4–13%). The fast method time facilitated a second quantitation for normal nucleosides at an appropriate dilution, allowing DNA damage concentrations to be contextualised accurately sample-to-sample. From DNA samples, the analytical processing time was < 8 h, and 96 samples can easily be prepared in a day. The method was used to quantify carbonyl, chloro- and oxo- adducts in murine tissue samples.

液相色谱质谱法分析核苷加合物是遗传毒性研究的有力工具。迄今为止的努力已经量化了一系列令人印象深刻的DNA损伤产物，尽管方法的多样性表明量化仍然是一项艰巨的任务。例如，正常核苷的不充分检查，繁琐的样品制备和大量的DNA需求被确定为经常出现的问题。提出了一种六分钟的超高效液相色谱方法，该方法可以从四个未修饰的核苷中充分分离出七个候选核苷加合物。该方法对每10 ⁸ 1加合物敏感正常碱基，大多数靶标的DNA输入量为20 µg。结果表明该方法是准确的（六种组织的五联体为81–119％）和精确的（相对标准偏差为4–13％）。快速的方法时间有助于在适当的稀释度下对正常核苷进行第二次定量，从而使DNA损伤浓度在不同样品之间准确地关联。从DNA样品中，分析处理时间少于8小时，并且一天之内就可以轻松制备96个样品。该方法用于定量鼠组织样品中的羰基，氯和氧加合物。