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Sensing of L -Arginine by Gut-Expressed Calcium Sensing Receptor Stimulates Gut Satiety Hormones Cholecystokinin and Glucose-Dependent Insulinotropic Peptide Secretion in Pig Model
Journal of Food Science ( IF 3.9 ) Pub Date : 2018-08-08 , DOI: 10.1111/1750-3841.14297
Chao Wang 1 , Cuicui Kang 1 , Yihan Xian 1 , Mingyu Zhang 1 , Xiaolin Chen 1 , Mingcai Pei 1 , Weiyun Zhu 1 , Suqin Hang 1
Affiliation  

Nutrients regulate the secretion of gut satiety hormones, which is related to the modulation of food intake and blood glucose levels. Calcium-sensing receptor (CaSR) is involved in regulating gut hormone secretion in response to l-amino acids and multivalent cations. Rodents are often used to investigate the effect of nutrients on these hormonal release. However, results obtained using rodent models are difficult to be applied in humans, we used pigs as a model in this study because their physiology is similar to that of humans. In this study, we investigated whether l-Arginine (l-Arg) could induce gut hormones cholecystokinin (CCK) and glucose-dependent insulinotropic peptide (GIP) secretion in the porcine duodenum and if so, whether CaSR mediated l-Arg-regulated gut satiety hormone secretion. Our data showed that treatment with 20 and 50 mM l-Arg induced CCK and GIP secretion compared with 0 mM l-Arg. However, treatment with d-Arg (an inactive isomer) failed to elicit this response. The potency of l-Arg to induce CCK and GIP secretion was enhanced in the presence of extracellular Ca2+ and CaSR agonist cinacalcet. However, the effect of Arg on CCK and GIP secretion was attenuated by blocking CaSR and its downstream signaling molecules adenylate cyclase (AC) and phospholipase C (PLC). Taken all together, pig duodenum provides an appropriate model to explore the effects of l-Arg on the secretion of the satiety-related gut hormones CCK and GIP and the role of CaSR in this effect. Further investigations are needed to verify the effect of l-Arg on food intake and blood glucose in human study. PRACTICAL APPLICATION: l-Arginine is able to modulate cholecystokinin and glucose-dependent insulinotropic peptide secretion through the CaSR in pig model, which has a potential role in regulating food intake and blood glucose levels.

中文翻译:

肠道表达的钙感应受体对 L-精氨酸的感应刺激猪模型中的肠道饱腹感激素缩胆囊素和葡萄糖依赖性促胰岛素肽分泌

营养素调节肠道饱腹感激素的分泌,这与调节食物摄入和血糖水平有关。钙敏感受体 (CaSR) 参与调节肠道激素分泌,以响应 l-氨基酸和多价阳离子。啮齿动物经常被用来研究营养素对这些激素释放的影响。然而,使用啮齿动物模型获得的结果很难应用于人类,我们在本研究中使用猪作为模型,因为它们的生理学与人类相似。在这项研究中,我们研究了 l-精氨酸 (l-Arg) 是否可以诱导猪十二指肠中的肠道激素缩胆囊素 (CCK) 和葡萄糖依赖性促胰岛素肽 (GIP) 分泌,如果是,CaSR 是否介导了 l-Arg 调节的肠道饱腹感荷尔蒙分泌。我们的数据显示,与 0 mM l-Arg 相比,20 和 50 mM l-Arg 处理诱导 CCK 和 GIP 分泌。然而,用 d-Arg(一种非活性异构体)处理未能引起这种反应。在细胞外 Ca2+ 和 CaSR 激动剂西那卡塞存在下,l-Arg 诱导 CCK 和 GIP 分泌的效力增强。然而,通过阻断 CaSR 及其下游信号分子腺苷酸环化酶 (AC) 和磷脂酶 C (PLC),可以减弱 Arg 对 CCK 和 GIP 分泌的影响。总之,猪十二指肠提供了一个合适的模型来探索 l-Arg 对饱腹感相关肠道激素 CCK 和 GIP 分泌的影响以及 CaSR 在这种影响中的作用。需要进一步的研究来验证 l-Arg 对人体研究中食物摄入和血糖的影响。实际应用:
更新日期:2018-08-08
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