Alkaline phosphatase (ALP), as an essential disease biomarker, widely distributes in various tissues, and plays a significant role in diagnosis and treatment of some diseases (e.g. cancers and liver dysfunction). Dichlorvos (DDVP), as one of typical organophosphate pesticide, could be hydrolyzed by ALP. In this work, we constructed a simple but robust 4-cyano-4′-pentylbiphenyl (5CB) droplet sensing platform for sensitive and convenient detection of DDVP based on its hydrolysis by ALP. The optical responses of liquid crystals (LCs) originated from controlling orientations of 5CB by using an ALP cleavable surfactant, sodium monododecyl phosphate (SMP). A dark crossed optical image of 5CB was observed due to formation of the SMP monolayer at the aqueous/LC droplet interface. Subsequently, 5CB turned to bright fan-shaped optical appearance upon addition of ALP and SMP mixture. Interestingly, LCs remained dark crossed optical images in contact with the pre-incubated mixture of DDVP and ALP. The detection limit of DDVP reached ~0.1 ng/mL. Isothermal titration calorimetry (ITC) and humam ALP ELISA kit provided strong evidence for the detection mechanism. This strategy was also used to evaluate whether the maximum pesticide residue limits of tomatoes conform to the relevant national standard requirement or not. Our study holds promise for real-time and label-free detection of pesticides with high sensitivity by employing portable LC-based sensing platform.

碱性磷酸酶（ALP）作为一种重要的疾病生物标志物，广泛分布于各种组织中，在某些疾病（例如癌症和肝功能障碍）的诊断和治疗中起着重要作用。敌敌畏（DDVP）是一种典型的有机磷酸酯农药，可以被ALP水解。在这项工作中，我们构建了一个简单但功能强大的4-氰基-4'-戊基联苯（5CB）液滴传感平台，用于基于ALP水解的DDVP的灵敏方便检测。液晶（LC）的光学响应源自通过使用ALP可裂解的表面活性剂单十二烷基磷酸钠（SMP）控制5CB的取向。由于在水/ LC液滴界面形成了SMP单层，因此观察到了5CB的暗交叉光学图像。随后，加入ALP和SMP混合物后5CB变成明亮的扇形光学外观。有趣的是，LCs与DDVP和ALP的预孵育混合物保持深色交叉的光学图像。DDVP的检出限达到〜0.1 ng / mL。等温滴定热法（ITC）和hummam ALP ELISA试剂盒为检测机制提供了有力的证据。该策略还用于评估番茄的最大农药残留限量是否符合相关的国家标准要求。我们的研究通过采用基于便携式LC的传感平台，有望以高灵敏度实时，无标签地检测农药。1 ng / mL。等温滴定热法（ITC）和humam ALP ELISA试剂盒为检测机制提供了有力的证据。该策略还用于评估番茄的最大农药残留限量是否符合相关的国家标准要求。我们的研究通过采用基于便携式LC的传感平台，有望以高灵敏度实时，无标签地检测农药。1 ng / mL。等温滴定热法（ITC）和hummam ALP ELISA试剂盒为检测机制提供了有力的证据。该策略还用于评估番茄的最大农药残留限量是否符合相关的国家标准要求。我们的研究通过采用基于便携式LC的传感平台，有望以高灵敏度实时，无标签地检测农药。