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3D printed flow-through cuvette insert for UV–Vis spectrophotometric and fluorescence measurements
Talanta ( IF 6.1 ) Pub Date : 2018-08-07 , DOI: 10.1016/j.talanta.2018.08.026
Michał Michalec , Łukasz Tymecki

Rapid Prototyping technologies expand the availability of fabrication of plastic objects to non-skilled users that need sophisticated equipment for their research. In this communication, for the very first time, the universal design of photometric-fluorometric, UV–Vis compatible, 3D-printed flow-through cuvette with two optical paths (2 and 10 mm) is introduced. The cuvette insert was made with the use of the most economically viable Fused Material Deposition technology which enables truly one-step manufacturing and easy replicating of the device. A utility of the cuvette was presented in the example of the basic flow injection analysis experiments on the model photometric (bromothymol blue) and fluorometric (fluorescein) dyes and proven by investigation of solubility constant of calcium hydrophosphate dihydrate by determination of phosphate using fluorescence quenching of molybdenum blue-Rhodamine B ion pair formation and calcium reaction with calcein in basic environment.



中文翻译:

用于紫外可见分光光度法和荧光测量的3D打印流通式比色皿插件

快速原型技术将塑料制品的制造范围扩展到需要熟练设备进行研究的非技术用户。在本次交流中,首次引入了具有两个光学路径(2和10 mm)的3D打印的流通池,即光度-荧光,UV-Vis兼容的通用设计。比色皿插件是使用最经济可行的熔融材料沉积技术制成的,该技术可实现真正的一步制造并易于复制该设备。

更新日期:2018-08-07
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