A novel nano-sized imprinted polymer/multi-walled carbon nanotube (MWCNTs)-based potentiometric sensor is introduced for lactic acid (LA) sensing in dairy products. The imprinted polymer was synthesized using allyl amine (AA) and ethylene glycol dimethacrylate as functional monomer and cross-linker, respectively. It was demonstrated that the amide linkage was created between LA and AA during copolymerization reaction which was finally hydrolyzed when removing template from the synthesized MIP. It was also shown that the MIP cavities, compatible with LA anion, were created during polymerization reaction which influenced the potentiometric response behavior of the MIP-based electrode. This novel potentiometric sensor is a carbon rod electrode, coated with a membrane consisting of the MIP nanoparticles (2.5%), MWCNTs (2%), dibutylphthalate (DBP) (65%), poly-vinyl chloride (PVC) (28.5%) and tetra phenyl phosphonium bromide (TPPB) (2%). The active ion sensed by the electrode is the LA anion formed at elevated pH condition. The sensor exhibited Nernstian slope of 30.3 ± 0.4 mVdecade⁻¹ in the working concentration range of 1.0 × 10⁻¹to 1.0 × 10⁻⁶ mol L⁻¹ with detection limit of 7.3 × 10⁻⁷ mol L⁻¹. The sensor displayed a stable potential response in the pH range of 5–8 and fast response time of less than 60 s. It exhibited also high selectivity over the interfering species. The proposed sensor was successfully applied for the determination of LA in real samples (milk and yoghurt).

一种新型的基于纳米压印的聚合物/多壁碳纳米管（MWCNTs）的电位传感器被引入，用于乳制品中的乳酸（LA）感测。分别使用烯丙胺（AA）和乙二醇二甲基丙烯酸酯作为功能单体和交联剂合成了印迹聚合物。证明在共聚反应期间在LA和AA之间建立了酰胺键，当从合成的MIP中除去模板时，该酰胺键最终被水解。还显示出在聚合反应期间产生了与LA阴离子相容的MIP腔，这影响了基于MIP的电极的电位响应行为。这种新型的电位传感器是碳棒电极，表面涂有由MIP纳米颗粒（2.5％），MWCNTs（2％），邻苯二甲酸二丁酯（DBP）（65％），聚氯乙烯（PVC）（28.5％）和四苯基溴化TP（TPPB）（2％）。电极检测到的活性离子是在升高的pH条件下形成的LA阴离子。传感器的Nernstian斜率为30.3±0.4 mV^-1中的1.0×10工作浓度范围^-1〜1.0×10 ^-6摩尔大号^-1与7.3×10检出限^-7摩尔大号^-1。该传感器在5–8的pH范围内显示出稳定的电位响应，而响应时间不到60 s。它对干扰物质也显示出高选择性。所提出的传感器已成功地用于测定实际样品（牛奶和酸奶）中的LA。