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Fate of lutein-containing zein nanoparticles following simulated gastric and intestinal digestion
Food Hydrocolloids ( IF 10.7 ) Pub Date : 2019-02-01 , DOI: 10.1016/j.foodhyd.2018.08.013
Christopher J. Cheng , Mario Ferruzzi , Owen G. Jones

Abstract Zein nanoparticles (ZNPs) were prepared to encapsulate lutein via solvent-induced nanoprecipitation, and the stability of the zein nanoparticles with encapsulated lutein (ZLNPs) was determined following in vitro gastric and intestinal simulation. Stability was assessed by dynamic light scattering, gel electrophoresis, tendency towards sedimentation, and both atomic force and light microscopy. ZNPs possessed a hydrodynamic radius of ∼75 nm, which was not altered with incorporation of lutein. Gastric digestion conditions induced significant aggregation and sedimentation of ZLNPs, which were not fully digested by gastric enzymes and were found adhered to lipid droplets in light micrographs. Aggregation was decreased and digestion was promoted during gastric digestion if salt was omitted, indicating that a high ion concentration increased ZLNP aggregation and limited enzymatic digestion. ZLNPs were redispersed in intestinal conditions and completely digested into peptides. In comparison to aqueous lutein dispersions, incorporation within ZNPs increased lutein's digestive stability by ∼58% but reduced its micellarization efficiency by ∼42%. These findings indicated that ZNPs provided a degree of physical protection to encapsulated carotenoids in gastric conditions yet might partially interfere with certain pathways for carotenoid bioaccessibility.

中文翻译:

模拟胃和肠道消化后含叶黄素的玉米醇溶蛋白纳米颗粒的命运

摘要 制备了玉米醇溶蛋白纳米粒(ZNPs)通过溶剂诱导的纳米沉淀包封叶黄素,并在体外胃和肠道模拟后确定了包封叶黄素(ZLNPs)的玉米醇溶蛋白纳米粒的稳定性。通过动态光散射、凝胶电泳、沉降趋势以及原子力和光学显微镜来评估稳定性。ZNPs 具有~75 nm 的流体动力学半径,它不会随着叶黄素的掺入而改变。胃消化条件导致 ZLNPs 显着聚集和沉淀,这些 ZLNPs 没有被胃酶完全消化,在光显微照片中发现粘附在脂滴上。如果不加盐,在胃消化过程中聚集减少并促进消化,表明高离子浓度增加了 ZLNP 聚集和有限的酶消化。ZLNPs 在肠道条件下重新分散并完全消化成肽。与叶黄素水分散体相比,在 ZNPs 中加入叶黄素的消化稳定性提高了约 58%,但其胶束化效率降低了约 42%。这些发现表明,ZNPs 为胃内包裹的类胡萝卜素提供了一定程度的物理保护,但可能会部分干扰类胡萝卜素生物可及性的某些途径。s 消化稳定性降低了约 58%,但其胶束化效率降低了约 42%。这些发现表明,ZNPs 为胃内包裹的类胡萝卜素提供了一定程度的物理保护,但可能会部分干扰类胡萝卜素生物可及性的某些途径。s 消化稳定性降低了约 58%,但其胶束化效率降低了约 42%。这些发现表明,ZNPs 为胃内包裹的类胡萝卜素提供了一定程度的物理保护,但可能会部分干扰类胡萝卜素生物可及性的某些途径。
更新日期:2019-02-01
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