Talanta ( IF 6.1 ) Pub Date : 2018-08-07 , DOI: 10.1016/j.talanta.2018.08.018 Shiyan Dai , Wei Zhang , Shuang Shao , Yujiao Tang , Lin Shao , Ting Liu , Guifang Cheng , Pingang He , Yuzhi Fang
Herein, a novel and convenient electrochemically active–inactive switching molecular beacon based on hemin (Hs-MB) has been designed for easy discrimination of single nucleotide polymorphisms (SNPs) and sensitive detection of insulin. The electrochemically active changing capability of Hs-MB is based on two identical hemin groups labeled at both ends of MB sequence in dimer or monomer forms depending on the conformation of MB which is in stem-loop structure or line shaped structure. The Hs-MB assay permits discrimination of SNPs and the highly sensitive and specific detection of insulin with detection limit successively as low as 0.5 pmol/L. Even at a very low target concentration, the Hs-MB assay also shows a good specificity in the presence of other potentially interfering components. The experimental results also show that Hs-MB can also be used for the accurate and rapid monitoring of insulin secretion by glucose-stimulated from MIN6 cells at different time periods, demonstrating that Hs-MB has potential in monitoring of biomarker variation in vivo.
中文翻译:
基于Hemin的新型电化学活性-惰性开关分子信标的设计策略,用于在均质溶液中直接进行SNP和胰岛素检测
在此,设计了一种基于血红素(Hs-MB)的新颖便捷的电化学活性-非活性开关分子信标,可轻松区分单核苷酸多态性(SNP)和灵敏地检测胰岛素。Hs-MB的电化学活性改变能力是基于两个相同的血红素基团,该两个血红素基团在MB序列的两端标记为二聚体或单体形式,取决于茎环结构或线形结构的MB的构象。Hs-MB检测可区分SNP,并能以低至0.5 pmol / L的检测限对胰岛素进行高度灵敏和特异的检测。即使在非常低的靶标浓度下,Hs-MB测定法在存在其他潜在干扰成分的情况下也显示出良好的特异性。