Ayahuasca is a psychoactive concoction prepared from the plants Banisteriopsis caapi and Psychotria viridis which are used ancestrally by Amazonian Indian populations and more recently, by Christian religious groups in Brazil and other countries. The aims of the present study were to identify the effects of ayahuasca on zebrafish embryo development and neurobehavior. Toxicity and developmental endpoints for zebrafish embryos were assessed from 0 to 1000 mg/L over 96 h of exposure. The effects on locomotor activity of zebrafish larvae were assessed using a video tracking system (ZebraBox) from 0 to 20 mg/L and after 120 and 144 h of exposure. The LC₅₀ of ayahuasca in zebrafish was determined as 236.3 mg/L. Ayahuasca exposure caused significant developmental anomalies in zebrafish embryos, mainly at the highest concentration tested, including hatching delay, loss of equilibrium, edema and the accumulation of red blood cells. Embryo behavior was also significantly affected, with decreased locomotor activity at the highest concentration tested. These results are in accordance with data obtained in mammal studies highlighting the possible risks of uncontrolled use of ayahuasca. Further research employing more specific behavior analysis could provide additional data on both therapeutic benefits and possible toxicological risk of ayahuasca.

阿亚瓦斯卡（Ayahuasca）是一种精神活性混合物，由植物Banisteriopsis caapi和Psychotria viridis制备而成，这些植物在祖先被亚马逊印第安人使用，最近也被巴西和其他国家的基督教宗教团体使用。本研究的目的是确定of鱼对斑马鱼胚胎发育和神经行为的影响。在暴露96小时后，斑马鱼胚胎的毒性和发育终点被评估为0至1000 mg / L。使用视频跟踪系统（ZebraBox）从0到20 mg / L以及暴露120和144小时后，评估了对斑马鱼幼虫运动能力的影响。LC ₅₀斑马鱼中阿育吠陀的含量为236.3 mg / L。Ayahuasca暴露在斑马鱼胚胎中引起了显着的发育异常，主要是在所测试的最高浓度下，包括孵化延迟，平衡失调，水肿和红细胞积聚。胚胎行为也受到显着影响，在最高测试浓度下运动能力下降。这些结果与在哺乳动物研究中获得的数据一致，突显了无管制使用阿育吠陀的可能风险。采用更具体的行为分析的进一步研究可以提供有关阿育吠陀的治疗益处和可能的毒理学风险的更多数据。