Invasive weeds ubiquitously found in terrestrial and aquatic ecosystems form potential feedstock for lignocellulosic ethanol production. The present study has reported a bioprocess for production of ethanol using mixed feedstock of 8 invasive weeds found in India. The feedstock was subjected to pretreatment comprising dilute acid hydrolysis (for hydrolysis of hemicellulosic fraction), alkaline delignification and enzymatic hydrolysis of cellulosic fraction. Pentose-rich and hexose-rich hydrolyzates obtained from pretreatment were fermented separately using microbial cultures of S. cerevisiae and C. shehatae. Fermentation mixture was sonicated at 35 kHz at 10% duty cycle. The time profiles of total reducing sugars, ethanol and biomass was fitted to a kinetic model using Genetic Algorithm. Sonication boosted the kinetics of fermentation 2-fold. The net bioethanol yield of the process was ∼220 g/kg raw biomass (with contributions of 86.8 and 133 g/kg raw biomass from pentose and hexose fermentations, respectively). Comparative evaluation of parameters of kinetic model under control and test conditions revealed several beneficial influences of sonication on both pentose and hexose fermentation systems such as faster transport of nutrients, substrate and products across cell membrane, rise in Monod saturation constant for substrate with concurrent reduction in substrate inhibition, and reduction of energy requirements for cell maintenance. Flow cytometry analysis of native and ultrasound-treated cells revealed no adverse influence of sonication on cell viability.

在陆地和水生生态系统中普遍发现的侵入性杂草构成了木质纤维素乙醇生产的潜在原料。本研究报告了一种利用印度发现的8种入侵杂草的混合原料生产乙醇的生物工艺。将原料进行预处理，该预处理包括稀酸水解（用于半纤维素级分的水解），碱去木质素和纤维素级分的酶促水解。使用酿酒酵母和Shehatae的微生物培养物分别发酵从预处理获得的富含戊糖和富含己糖的水解产物。。发酵混合物在35 kHz下以10％的占空比进行超声处理。使用遗传算法将总还原糖，乙醇和生物质的时间曲线拟合为动力学模型。超声处理使发酵动力学提高了2倍。该工艺的净生物乙醇产量约为220 g / kg原始生物质（戊糖和己糖发酵分别贡献了86.8和133 g / kg原始生物质）。在控制和测试条件下动力学模型参数的比较评估表明，超声处理对戊糖和己糖发酵系统都有一些有益的影响，例如营养物质，底物和产物在细胞膜上的更快运输，底物的Monod饱和常数的增加以及同时降低。抑制底物，降低细胞维持所需的能量。