An affinity sensor based on capacitive transduction was developed to detect benzo(a)pyrene (BaP) in river water. Two types of recognition elements, the synthetic receptor analogues molecularly imprinted polymers (MIPs) and natural monoclonal antibody (mAb) were tested for this type of biosensor. Different polymerization strategies to obtain MIPs were compared. One approach comprised a preliminary batch synthesis of beads that were subsequently coupled covalently to an electrode surface. Another approach consisted of the in-situ synthesis of MIPs directly onto the electrode surface using electropolymerization. The latter approach provided the best results. To choose the optimal recognition element mAb and MIP-modified electrodes different sets were evaluated with regards to their sensitivity, selectivity, linear range and re-usability. The mAb-modified electrodes were considerably more sensitive toward BaP (ng L⁻¹ range vs µg L⁻¹ range for the MIP-modified one), and showed a broader linear working range than the MIP-modified electrodes. The latter revealed more suitable for group-selective determination of PAHs. The developed capacitive sensor was tested for the detection of BaP in naturally-contaminated water samples collected in different places of Ghent, Belgium. The results obtained with the sensor were coherent and reproducible, and were in a good agreement with the confirmation technique, HPLC-FlD.

开发了一种基于电容转导的亲和力传感器来检测河水中的苯并（a）re（BaP）。对于这种类型的生物传感器，测试了两种类型的识别元件，即合成受体类似物分子印迹聚合物（MIP）和天然单克隆抗体（mAb）。比较了获得MIP的不同聚合策略。一种方法包括珠的初步批量合成，其随后共价偶联至电极表面。另一种方法包括使用电聚合将MIP直接原位合成到电极表面上。后一种方法提供了最佳结果。为了选择最佳识别元件mAb和MIP修饰的电极，对它们的灵敏度，选择性，线性范围和可重复使用性进行了评估。MIP修饰电极的^-1范围相对于µg L ^-1范围，并且显示出比MIP修饰电极更宽的线性工作范围。后者显示更适合组选择性测定PAHs。对开发的电容式传感器进行了测试，以检测比利时根特不同地方收集的自然污染水样品中的BaP。用传感器获得的结果是连贯且可重复的，并且与确认技术HPLC-FID很好地吻合。