Abstract Universal Biomarker Analysis is a GC×GC-ToFMS method that provides absolute quantification and non-targeted pseudo MS-MS for all resolved hydrocarbon components in petroleum and rock extracts. This is achieved by merging two chromatographic datasets obtained under field ionization (FI), which provides molecular ions, and electron impact (EI), which provides diagnostic fragment ions. We recently demonstrated this technique for the analysis of saturated biomarkers (Walters et al., 2018). Here, we show its application to aromatic biomarkers. Universal Biomarker Analysis is compared to conventional GC–MS for frequently analyzed aromatic hydrocarbons, such as the alkylated naphthalenes and biphenyls, mono- and tri-aromatic steroidal hydrocarbons to illustrate where this new method provides demonstrated advantages. The technique is well suited for the analysis of rare species not commonly analyzed in targeted GC–MS/MS analyses. This is illustrated by the tentative identification of A/B-ring C26-C29 diaromatic steroidal hydrocarbons in a crude oil, compounds that have been previously identified as minor components of immature rock extracts, and indications of previously unknown C26 methyl-triaromatic steroidal hydrocarbons.

中文翻译：

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通用生物标志物分析：芳烃

摘要 通用生物标志物分析是一种 GC×GC-ToFMS 方法，可为石油和岩石提取物中所有已解析的烃组分提供绝对定量和非靶向伪 MS-MS。这是通过合并在提供分子离子的场电离 (FI) 和提供诊断碎片离子的电子碰撞 (EI) 下获得的两个色谱数据集来实现的。我们最近展示了这种用于分析饱和生物标志物的技术（Walters 等，2018）。在这里，我们展示了它在芳香生物标志物上的应用。通用生物标志物分析与常规 GC-MS 对经常分析的芳烃（如烷基化萘和联苯、单和三芳族甾体烃）进行比较，以说明这种新方法在哪些方面具有明显的优势。该技术非常适用于在靶向 GC-MS/MS 分析中不常分析的稀有物种的分析。这通过对原油中 A/B 环 C26-C29 二芳族甾体烃的初步鉴定、先前已被鉴定为未成熟岩石提取物的次要成分的化合物以及先前未知的 C26 甲基三芳烃甾体烃的迹象来说明。