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Comparison between the 3M MDS® method and phenotypic methods to detect Salmonella spp. in foods
LWT - Food Science and Technology ( IF 6 ) Pub Date : 2018-07-30 , DOI: 10.1016/j.lwt.2018.07.061
Greici Bergamo , Cláudio Dias Timm , Natália Rodrigues Carvalho , Elizabete Helbig , Eliezer Avila Gandra

The objective of this study was to compare three methods for detection of Salmonella spp., the first recommended by the Brazilian Ministry of Agriculture, Livestock and Supply - known as MAPA -, the second described by Annex D of ISO 6579 - known as MSRV and the third method based on molecular techniques known as 3M MDS®. The assays were performed using samples of artificially contaminated UHT milk (101–103 CFU - Test), and mixed-meat and pork sausages commercialized in southern Brazil. MAPA, MSRV and 3M MDS® methods present a sensitivity of 100%, 100% and 97.6%, respectively. The specificity was 100% for the three methods. The data shows that 3M MDS® and MSRV are reliable methods because they revealed equivalent sensitivity and specificity to the Brazilian official method (MAPA) and that possibly can be used as a viable alternative for the detection of Salmonella spp.



中文翻译:

3M的MDS之间的比较®方法和表型的方法来检测沙门氏菌属。在食物中

这项研究的目的是比较三种检测沙门氏菌的方法,第一种方法是巴西农业,畜牧与供应部推荐的-称为MAPA-第二种方法是由ISO 6579的附录D描述的-称为MSRV和基于被称为3M MDS分子技术第三方法®。使用人工污染的UHT牛奶(10 1 – 10 3  CFU-测试)样品以及在巴西南部商业化的混合肉和猪肉香肠样品进行测定。MAPA,MSRV和3M MDS ®方法呈现的分别为100%,100%和97.6%,灵敏度。三种方法的特异性均为100%。数据显示,3M MDS ®MSRV和MSRV是可靠的方法,因为它们显示出与巴西官方方法(MAPA)同等的敏感性和特异性,并且可以用作检测沙门氏菌的可行替代方法。

更新日期:2018-07-31
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