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Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films
Biomacromolecules ( IF 6.2 ) Pub Date : 2018-07-27 00:00:00 , DOI: 10.1021/acs.biomac.8b00626
Antonio Francesko 1 , Kristina Ivanova 1 , Javier Hoyo 1 , Sílvia Pérez-Rafael 1 , Petya Petkova 1 , Margarida M Fernandes 1 , Thomas Heinze 2 , Ernest Mendoza 3 , Tzanko Tzanov 1
Affiliation  

In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA). At first, dispersions of AgNPs decorated with chitosan (CS) or aminocellulose (AC) were synthesized by applying high intensity ultrasound. These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element. SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers. The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli. Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs. Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.

中文翻译:

自下而上的抗菌独立式纳米生物复合膜的层层组装。

在这项研究中,通过依次沉积生物聚合物封端的银纳米颗粒(AgNPs)和透明质酸(HA)获得了独立的纳米生物复合膜。首先,通过应用高强度超声合成了用壳聚糖(CS)或氨基纤维素(AC)装饰的AgNP的分散体。这些聚阳离子纳米实体与HA聚阴离子逐层组装,以生成稳定的3D超分子构建体,其中生物聚合物封端的AgNPs发挥活性剂和结构元素的双重作用。组件的SEM图像显示厚度随沉积双层数的增加而逐渐增加。≥50双层的复合材料对人体细胞安全,对金黄色葡萄球菌大肠杆菌具有100%的抗菌活性。此外,含有CSAgNPs的薄膜完全防止了生物膜的形成,从而使细胞表面的粘附性降低了多达6个对数。这种纳米生物复合材料可以作为控制细菌在受伤的皮肤,烧伤和慢性伤口上生长的有效屏障。
更新日期:2018-07-27
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