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Characterization of Naïve and Vitamin C-Treated Mouse Schwann Cell Line MSC80: Induction of the Antioxidative Thioredoxin Related Transmembrane Protein 1
Journal of Proteome Research ( IF 4.4 ) Pub Date : 2018-08-10 , DOI: 10.1021/acs.jproteome.8b00022
Vietxuan Phan 1 , Jens Schmidt 2 , Vitali Matyash 3 , Sebastian Malchow 1 , Michaela Thanisch 2 , Christin Lorenz 1 , Irmgard Diepolder 2 , Jörg Bernhard Schulz 2, 4 , Werner Stenzel 3 , Andreas Roos 1 , Burkhard Gess 2
Affiliation  

Schwann cells (SCs) are essential in the production of the axon-wrapping myelin sheath and provide trophic function and repair mechanisms in the peripheral nerves. Consequently, well-characterized SC in vitro models are needed to perform preclinical studies including the investigation of the complex biochemical adaptations occurring in the peripheral nervous system (PNS) under different (patho)physiological conditions. MSC80 cells represent a murine SC line used as an in vitro system for neuropathological studies. Here, we introduce the most abundant 9532 proteins identified via mass spectrometry-based protein analytics, and thus provide the most comprehensive SC protein catalogue published thus far. We cover proteins causative for inherited neuropathies and demonstrate that in addition to cytoplasmic, nuclear and mitochondrial proteins and others belonging to the protein processing machinery are very well covered. Moreover, we address the suitability of MSC80 to examine the molecular effect of a drug-treatment by analyzing the proteomic signature of Vitamin C-treated cells. Proteomic findings, immunocytochemistry, immunoblotting and functional experiments support the concept of a beneficial role of Vitamin C on oxidative stress and identified TMX1 as an oxidative stress protective factor, which might represent a promising avenue for therapeutic intervention of PNS-disorders with oxidative stress burden such as diabetic neuropathy.

中文翻译:

幼稚和维生素C处理的小鼠雪旺细胞系MSC80的表征:抗氧化硫氧还蛋白相关跨膜蛋白1的诱导。

雪旺氏细胞(SCs)在产生轴突包裹的髓鞘的过程中至关重要,并在周围神经中提供营养功能和修复机制。因此,需要进行充分表征的SC体外模型来进行临床前研究,包括研究在不同(病理)生理条件下外周神经系统(PNS)中发生的复杂生化适应。MSC80细胞代表一种鼠类SC系,用作神经病理学研究的体外系统。在这里,我们介绍了通过基于质谱的蛋白质分析鉴定出的最丰富的9532蛋白质,从而提供了迄今为止发布的最全面的SC蛋白质目录。我们涵盖了引起遗传性神经病的蛋白质,并证明了除了细胞质外,核蛋白和线粒体蛋白以及其他属于蛋白加工机器的蛋白都被很好地涵盖了。此外,我们通过分析经维生素C处理的细胞的蛋白质组学特征,探讨了MSC80检验药物治疗的分子效应的适用性。蛋白质组学发现,免疫细胞化学,免疫印迹和功能性实验均支持维生素C对氧化应激的有益作用的概念,并确定TMX1为氧化应激保护因子,这可能代表了治疗性干预PNS疾病的氧化应激负担的途径,例如作为糖尿病性神经病。我们通过分析经维生素C处理的细胞的蛋白质组学特征,探讨了MSC80检查药物治疗的分子效应的适用性。蛋白质组学发现,免疫细胞化学,免疫印迹和功能性实验均支持维生素C对氧化应激的有益作用的概念,并确定TMX1为氧化应激保护因子,这可能代表了治疗性干预PNS疾病的氧化应激负担的途径,例如作为糖尿病性神经病。我们通过分析经维生素C处理的细胞的蛋白质组学特征,探讨了MSC80检查药物治疗的分子效应的适用性。蛋白质组学发现,免疫细胞化学,免疫印迹和功能性实验均支持维生素C对氧化应激的有益作用的概念,并确定TMX1为氧化应激保护因子,这可能代表了治疗性干预PNS疾病的氧化应激负担的途径,例如作为糖尿病性神经病。
更新日期:2018-08-10
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