Hispidulin, a natural flavone, has been reported to have diverse pharmacological effects, including antifungal, antioxidant, and antithrombotic properties. However, an anti-adipogenic effect has not yet been reported, which is the focus of the current study. Hispidulin suppressed the differentiation of adipocytes and cellular lipid accumulation without cytotoxicity. Treatment with hispidulin at concentrations of 10, 20, and 40 μM reduced intracellular lipids by 88.1%, 81.9%, and 75.8%, respectively. In addition, hispidulin reduced mRNA and protein expression of peroxisome proliferator-activated receptor gamma (PPARγ) and adiponectin. To our knowledge, these results are the first evidence of the anti-adipogenic effects of hispidulin in 3T3-L1 adipocytes, indicating that hispidulin has potential as a novel anti-obesity therapeutic.

据报道，Hispidulin是一种天然的黄酮，具有多种药理作用，包括抗真菌，抗氧化剂和抗血栓形成特性。然而，尚未报道抗脂肪形成作用，这是当前研究的重点。Hispidulin抑制脂肪细胞的分化和细胞脂质蓄积，而无细胞毒性。用浓度为10、20和40μM的组织蛋白处理可以分别减少88.1％，81.9％和75.8％的细胞内脂质。此外，组蛋白降低过氧化物酶体增殖物激活受体γ（PPARγ）和脂联素的mRNA和蛋白表达。据我们所知，这些结果是组蛋白在3T3-L1脂肪细胞中抗脂肪形成作用的第一个证据，表明组蛋白具有作为新型抗肥胖治疗剂的潜力。