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Avidin Adsorption to Silk Fibroin Films as a Facile Method for Functionalization
Biomacromolecules ( IF 6.2 ) Pub Date : 2018-07-24 00:00:00 , DOI: 10.1021/acs.biomac.8b00824
Alycia Abbott 1 , Leif Oxburgh 2 , David L. Kaplan 3 , Jeannine M. Coburn 1, 3
Affiliation  

Silk fibroin biomaterials are highly versatile in terms of materials formation and functionalization, with applications in tissue engineering and drug delivery, but necessitate modifications for optimized biological activity. Herein, a facile, avidin-based technique is developed to noncovalently functionalize silk materials with bioactive molecules. The ability to adsorb avidin to silk surfaces and subsequently couple biotinylated macromolecules via avidin–biotin interaction is described. This method better preserved functionality than standard covalent coupling techniques using carbodiimide cross-linking chemistry. The controlled release of avidin from the silk surface was demonstrated by altering the adsorption parameters. Application of this technique to culturing human foreskin fibroblasts (hFFs) and human mesenchymal stem cells (hMSCs) on arginine-glycine-aspartic-acid-modified (RGD-modified) silk showed increased cell growth over a seven-day period. This technique provides a facile method for the versatile functionalization of silk materials for biomedical applications including tissue engineering, drug delivery, and biological sensing.

中文翻译:

亲和素吸附到丝素蛋白膜上作为一种功能化的简便方法

丝素蛋白生物材料在材料形成和功能化方面具有广泛的用途,可应用于组织工程和药物输送,但必须进行修饰以优化生物活性。在此,开发了一种基于亲和素的简便技术,以利用生物活性分子非共价地官能化丝绸材料。描述了将亲和素吸附到丝表面并随后通过亲和素-生物素相互作用偶联生物素化的大分子的能力。与使用碳二亚胺交联化学的标准共价偶联技术相比,该方法可更好地保留功能。通过改变吸附参数可以证明亲和素从蚕丝表面的控制释放。该技术在精氨酸-甘氨酸-天冬氨酸-修饰的(RGD-修饰的)丝上培养人包皮成纤维细胞(hFFs)和人间充质干细胞(hMSCs)的应用显示在七天的时间内细胞生长增加。该技术为丝绸材料的多功能功能化提供了一种简便的方法,以用于生物医学应用,包括组织工程,药物输送和生物传感。
更新日期:2018-07-24
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