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In vitro meningeal permeation of MnFe2O4 nanoparticles
Chemico-Biological Interactions ( IF 5.1 ) Pub Date : 2018-07-24 , DOI: 10.1016/j.cbi.2018.07.020
Marcella Mauro , Matteo Crosera , Massimo Bovenzi , Gianpiero Adami , Elena Baracchini , Giovanni Maina , Francesca Larese Filon

MnFe2O4 nanoparticles (NPs) are commonly produced in some occupational settings and may reach high concentration in activities such as arc-welding or ferroalloy metallurgy. Manganese is an essential cofactor in enzyme activities but it has been demonstrated that long-term exposure to excessive levels can lead to “manganism”, a neurodegenerative disease resembling Parkinson features.

Inhaled NPs deposit partially in pharynx and nasopharynx and may reach the central nervous system through the olfactory nerve, which is completely enveloped by the meningeal membranes throughout its course from the nasal cavity to the olfactory bulb or through the trigeminal nerves. This study investigated in vitro the transmeningeal absorption of 50 nm MnFe2O4NPs, using excised porcine meninges mounted on Franz diffusion cells. We tested two donor solutions: the first containing MnFe2O4NPs (2.0 g/L) and the second obtained by the ultrafiltration of the first one, in order to test only the NPs water soluble fraction. Each experiment was carried separately for 4 h. Results showed that no Mn flux permeation through the meninges occurred, since only trace of the metal was found in receivers solutions of cells exposed to MnFeNPs (5.5 ± 2.2 ng/cm2), ultrafiltered solution (3.5 ± 1.5 ng/cm2) and blank cells. (2.1 ± 0.6 ng/cm2) (mean and SE). Differences did not reach the statistical significance.

Our study shows – for the first time - that MnFe2O4NPs penetrate the meningeal membrane in a negligible amount, thus making unlikely the hypothesis of a transcellular and paracellular absorption through the olfactory nerve but not excluding the hypothesis of an active intraneuronal absorption.



中文翻译:

MnFe 2 O 4纳米粒子的体外脑膜渗透

MnFe 2 O 4纳米颗粒(NPs)通常在某些职业环境中生产,并可能在电弧焊或铁合金冶金等活动中达到很高的浓度。锰是酶活性中必不可少的辅助因子,但事实证明,长期暴露于过量的锰会导致“锰”,这是一种类似于帕金森氏症的神经退行性疾病。

吸入的NPs部分沉积在咽和鼻咽中,并可能通过嗅神经到达中枢神经系统,嗅神经在从鼻腔到嗅球或整个三叉神经的整个过程中被脑膜完全包裹。这项研究使用安装在Franz扩散池上的切除的猪脑膜对50 nm MnFe 2 O 4 NPs的经脑吸收进行了体外研究。我们测试了两种供体溶液:第一种包含MnFe 2 O 4NPs(2.0 g / L),第二种是通过超滤获得的,第一种是仅测试NPs的水溶性级分。每个实验分别进行4小时。结果表明,由于在暴露于MnFeNPs(5.5±2.2 ng / cm 2),超滤溶液(3.5±1.5 ng / cm 2)和空白单元格。(2.1±0.6 ng / cm 2)(平均值和标准误差)。差异未达到统计意义。

我们的研究首次显示MnFe 2 O 4 NPs的渗透量可忽略不计,因此不可能通过嗅觉神经进行跨细胞和副细胞吸收的假设,但不能排除主动神经内吸收的假设。

更新日期:2018-07-24
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