Chemicals exert harmful effects on target species and cause DNA damage after their exposure. Here, Au–Ag heterodimers have been fabricated with conjugated molecules such as DNA, cell-penetrating peptide, and thiolated polyethylene glycol to investigate the intracellular interactions between fipronil and neuron cells. The hybridized heterodimer nanoparticles show a strong plasmonic circular dichroism signal and were used for the intracellular detection of fipronil-induced 8-hydroxy-2′-deoxyguanosine (8-OHdG), and the limit of detection for 8-OHdG was as low as 0.1 nM. More importantly, 8-OHdG could be visualized in cells with in situ cell imaging based on luminescence quenching via energy transfer. This method has been applied to measure the oxidative DNA damage caused by nonylphenol and alternariol in cells. All our results suggest that our chiroplasmonic platform is a promising and applicable method not only for detecting the 8-OHdG induced by fipronil in cells but also for assessing the capacity of chemicals to induce intracellular oxidative DNA damage.

中文翻译：

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Fipronil与神经元细胞之间的循环二色性-主动相互作用

化学物质对目标物种产生有害影响，并在暴露后造成DNA损伤。在这里，Au-Ag异二聚体已经与诸如DNA，穿透细胞的肽和硫醇化的聚乙二醇等共轭分子一起制成，以研究氟虫腈和神经元细胞之间的细胞内相互作用。杂交的异二聚体纳米颗粒显示强等离子圆二色性信号，并用于氟虫腈诱导的8-羟基-2'-脱氧鸟苷（8-OHdG）的细胞内检测，并且8-OHdG的检测限低至0.1 nM。更重要的是，可以原位观察细胞中的8-OHdG基于通过能量转移的发光淬灭的细胞成像。该方法已用于测量细胞中壬基酚和交替烯酚引起的氧化性DNA损伤。所有我们的结果表明，我们的手性等离子体平台是一种有前途且可应用的方法，不仅可用于检测氟虫腈在细胞中诱导的8-OHdG，而且可用于评估化学物质诱导细胞内氧化性DNA损伤的能力。