The practical use of additive manufacturing to create artificial bone as a material for repairing complex bone defects is currently attracting attention. In this study, we compared the osteogenic capacity of materials composited by the method developed by Kokubo et al. of treating 3D-printed titanium (Ti) mesh with a mixture of H₂SO₄ and HCl and heating (mixed-acid and heat treatment) with that of materials subjected to conventional chemical treatment. Ti plates treated with this method have been found to promote highly active bone formation on their surface when inserted into rabbit tibial bone defects. No previous study has compared this method with other surface treatment methods. In this study, we used histological and other observations to compare the bone formation process in bone defects when Ti meshes prepared by the selective laser melting technique (SLM) and treated either with mixed acids and heat or with conventional chemical Ti surface treatments were implanted in a rat calvarial bone defect model. We found that both micro-computed tomography and observations of undecalcified ground sections showed that the best bone formation was observed in rats implanted with mesh treated with mixed acids and heat. Our results suggest that mixed-acid and heat-treated Ti mesh prepared by SLM may have a high osteogenic capacity in bone defects.

中文翻译：

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激光选区熔融技术制备混合酸热处理钛网的成骨能力

目前，增材制造作为修复复杂骨缺损材料的人工骨的实际应用受到关注。在这项研究中，我们比较了 Kokubo等人开发的方法复合材料的成骨能力。_{用 H 2} SO ₄的混合物处理 3D 打印的钛 (Ti) 网和经过常规化学处理的材料的 HCl 和加热（混合酸和热处理）。已发现用这种方法处理过的钛板在插入兔胫骨缺损处时可促进其表面的高活性骨形成。以前没有研究将这种方法与其他表面处理方法进行比较。在这项研究中，我们使用组织学和其他观察结果比较了当通过选择性激光熔化技术 (SLM) 制备并用混合酸和热或常规化学 Ti 表面处理方法处理的 Ti 网时，骨缺损中的骨形成过程。大鼠颅骨缺损模型。我们发现，微计算机断层扫描和未脱钙地面切片的观察表明，在植入了混合酸和热处理的网片的大鼠中观察到了最佳的骨形成。我们的研究结果表明，由 SLM 制备的混合酸和热处理的钛网在骨缺损中可能具有高成骨能力。